Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Moriguchi, T.; Ida, K.; Hikima, T.; Ueno, G.; Yamamoto, M.; Suzuki, H.
    Channeling and conformational changes in the heterotetrameric sarcosine oxidase from Corynebacterium sp. U-96 (2010), J. Biochem., 148, 491-505 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.3.24 expressed in Escherichia coli BL21(DE3) cells Corynebacterium sp. U-96

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.5.3.24 enzyme complexed with methylthioacetate, pyrrole 2-carboxylate and sulfite or sarcosine-reduced enzyme, using 0.1 M HEPES (pH 7.5) and 1.7 M ammonium sulfate Corynebacterium sp. U-96

Protein Variants

EC Number Protein Variants Comment Organism
1.5.3.24 Y269A the mutant shows 10% activity compared to the wild type enzyme Corynebacterium sp. U-96
1.5.3.24 Y271A the mutant shows 0.0076% activity compared to the wild type enzyme Corynebacterium sp. U-96
1.5.3.24 Y271F the mutant shows 0.78% activity compared to the wild type enzyme Corynebacterium sp. U-96

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.3.24 methylthioacetate competitive inhibition with respect to sarcosine Corynebacterium sp. U-96
1.5.3.24 pyrrole 2-carboxylate competitive inhibition with respect to sarcosine Corynebacterium sp. U-96

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.3.24 1.4
-
 sarcosine wild type enzyme, at pH 8.0 and 25°C Corynebacterium sp. U-96
1.5.3.24 11.3
-
 sarcosine mutant enzyme Y269A, at pH 8.0 and 25°C Corynebacterium sp. U-96
1.5.3.24 72.6
-
 sarcosine mutant enzyme Y271F, at pH 8.0 and 25°C Corynebacterium sp. U-96
1.5.3.24 1730
-
 sarcosine mutant enzyme Y271A, at pH 8.0 and 25°C Corynebacterium sp. U-96

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.3.24 sarcosine + 5,6,7,8-tetrahydrofolate + O2 Corynebacterium sp. U-96
-
 glycine + 5,10-methylenetetrahydrofolate + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.3.24 Corynebacterium sp. U-96
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.3.24 sarcosine + 5,6,7,8-tetrahydrofolate + O2
-
 Corynebacterium sp. U-96 glycine + 5,10-methylenetetrahydrofolate + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.3.24 heterotetramer 1 * 110000 + 1 * 44000 + 1 * 21000 + 1 * 10000, SDS-PAGE Corynebacterium sp. U-96

Synonyms

EC Number Synonyms Comment Organism
1.5.3.24 heterotetrameric sarcosine oxidase
-
 Corynebacterium sp. U-96
1.5.3.24 sarcosine:oxygen oxidoreductase (demethylating)
-
 Corynebacterium sp. U-96

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5.3.24 1.65
-
 sarcosine mutant enzyme Y271A, at pH 8.0 and 25°C Corynebacterium sp. U-96
1.5.3.24 7.15
-
 sarcosine mutant enzyme Y271F, at pH 8.0 and 25°C Corynebacterium sp. U-96
1.5.3.24 14.5
-
 sarcosine mutant enzyme Y269A, at pH 8.0 and 25°C Corynebacterium sp. U-96
1.5.3.24 17.5
-
 sarcosine wild type enzyme, at pH 8.0 and 25°C Corynebacterium sp. U-96

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.3.24 FAD
-
 Corynebacterium sp. U-96
1.5.3.24 FMN
-
 Corynebacterium sp. U-96

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.5.3.24 0.33
-
 pyrrole 2-carboxylate at pH 8.0 and 25°C Corynebacterium sp. U-96
1.5.3.24 2.05
-
 methylthioacetate at pH 8.0 and 25°C Corynebacterium sp. U-96

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.5.3.24 0.00095
-
 sarcosine mutant enzyme Y271A, at pH 8.0 and 25°C Corynebacterium sp. U-96
1.5.3.24 0.098
-
 sarcosine mutant enzyme Y271F, at pH 8.0 and 25°C Corynebacterium sp. U-96
1.5.3.24 1.3
-
 sarcosine mutant enzyme Y269A, at pH 8.0 and 25°C Corynebacterium sp. U-96
1.5.3.24 12.5
-
 sarcosine wild type enzyme, at pH 8.0 and 25°C Corynebacterium sp. U-96