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Literature summary extracted from
- Soluble expression of Thermomicrobium roseum sarcosine oxidase and characterization of N-demethylation activity (2019), Mol. Catal., 464, 48-56 .
No PubMed abstract available
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.5.3.1 | expression in Bacillus subtilis | Thermomicrobium roseum |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.5.3.1 | 0.25 | - |
N-Methyl-L-alanine | wild-type, 37°C, pH not specified in the publication | Thermomicrobium roseum |  |
| 1.5.3.1 | 61.2 | - |
sarcosine | wild-type, 37°C, pH not specified in the publication | Thermomicrobium roseum | |
| 1.5.3.1 | 98 | - |
L-proline | wild-type, 37°C, pH not specified in the publication | Thermomicrobium roseum | |
| 1.5.3.1 | 136.5 | - |
N-methyl-L-tryptophan | wild-type, 37°C, pH not specified in the publication | Thermomicrobium roseum | |
| 1.5.3.1 | 154.3 | - |
2-isopropyl-4-(methylaminomethyl)thiazole | wild-type, 37°C, pH not specified in the publication | Thermomicrobium roseum | |
| 1.5.3.1 | 168.5 | - |
N-Methyl-L-leucine | wild-type, 37°C, pH not specified in the publication | Thermomicrobium roseum | |
| 1.5.3.1 | 177.8 | - |
N-methyl-L-phenylalanine | wild-type, 37°C, pH not specified in the publication | Thermomicrobium roseum | |
| 1.5.3.1 | 244.7 | - |
N-methyl-L-aspartate | wild-type, 37°C, pH not specified in the publication | Thermomicrobium roseum | |
| 1.5.3.1 | 258.4 | - |
4-(ethylaminomethyl)pyridine | wild-type, 37°C, pH not specified in the publication | Thermomicrobium roseum | |
| 1.5.3.1 | 324.5 | - |
N-methyl-D-proline | wild-type, 37°C, pH not specified in the publication | Thermomicrobium roseum |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.5.3.1 | Thermomicrobium roseum | B9L163 | - |
- |
| 1.5.3.1 | Thermomicrobium roseum DSM 5159 | B9L163 | - |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.5.3.1 | 2-isopropyl-4-(methylaminomethyl)thiazole + O2 + H2O | - |
Thermomicrobium roseum | formaldehyde + 2-isopropyl-4-(aminomethyl)thiazole + H2O2 | - |
? | |
| 1.5.3.1 | 2-isopropyl-4-(methylaminomethyl)thiazole + O2 + H2O | - |
Thermomicrobium roseum DSM 5159 | formaldehyde + 2-isopropyl-4-(aminomethyl)thiazole + H2O2 | - |
? | |
| 1.5.3.1 | 4-(ethylaminomethyl)pyridine + O2 + H2O | - |
Thermomicrobium roseum | acetaldehyde + 4-(aminomethyl) pyridine + H2O2 | - |
? | |
| 1.5.3.1 | 4-(ethylaminomethyl)pyridine + O2 + H2O | - |
Thermomicrobium roseum DSM 5159 | acetaldehyde + 4-(aminomethyl) pyridine + H2O2 | - |
? | |
| 1.5.3.1 | L-proline + O2 + H2O | - |
Thermomicrobium roseum | ? + H2O2 | - |
? | |
| 1.5.3.1 | N-methyl-D-proline + O2 + H2O | - |
Thermomicrobium roseum | formaldehyde + D-proline + H2O2 | - |
? | |
| 1.5.3.1 | N-methyl-L-alanine + O2 + H2O | - |
Thermomicrobium roseum | formaldehyde + L-alanine + H2O2 | - |
? | |
| 1.5.3.1 | N-methyl-L-alanine + O2 + H2O | - |
Thermomicrobium roseum DSM 5159 | formaldehyde + L-alanine + H2O2 | - |
? | |
| 1.5.3.1 | N-methyl-L-aspartate + O2 + H2O | - |
Thermomicrobium roseum | formaldehyde + L-aspartate + H2O2 | - |
? | |
| 1.5.3.1 | N-methyl-L-leucine + O2 + H2O | - |
Thermomicrobium roseum | formaldehyde + L-leucine + H2O2 | - |
? | |
| 1.5.3.1 | N-methyl-L-leucine + O2 + H2O | - |
Thermomicrobium roseum DSM 5159 | formaldehyde + L-leucine + H2O2 | - |
? | |
| 1.5.3.1 | N-methyl-L-phenylalanine + O2 + H2O | - |
Thermomicrobium roseum | formaldehyde + L-phenylalanine + H2O2 | - |
? | |
| 1.5.3.1 | N-methyl-L-tryptophan + O2 + H2O | - |
Thermomicrobium roseum | formaldehyde + L-tryptophan + H2O2 | - |
? | |
| 1.5.3.1 | sarcosine + O2 + H2O | - |
Thermomicrobium roseum | formaldehyde + glycine + H2O2 | - |
? | |
| 1.5.3.1 | sarcosine + O2 + H2O | - |
Thermomicrobium roseum DSM 5159 | formaldehyde + glycine + H2O2 | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.5.3.1 | ? | x * 43000, SDS-PAGE | Thermomicrobium roseum |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.5.3.1 | 100 | - |
melting temperature | Thermomicrobium roseum |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.5.3.1 | 0.00004 | - |
4-(ethylaminomethyl)pyridine | wild-type, 37°C, pH not specified in the publication | Thermomicrobium roseum | |
| 1.5.3.1 | 0.0003 | - |
L-proline | wild-type, 37°C, pH not specified in the publication | Thermomicrobium roseum | |
| 1.5.3.1 | 0.004 | - |
N-Methyl-L-alanine | wild-type, 37°C, pH not specified in the publication | Thermomicrobium roseum | |
| 1.5.3.1 | 0.0045 | - |
N-methyl-L-tryptophan | wild-type, 37°C, pH not specified in the publication | Thermomicrobium roseum | |
| 1.5.3.1 | 0.0048 | - |
N-methyl-D-proline | wild-type, 37°C, pH not specified in the publication | Thermomicrobium roseum | |
| 1.5.3.1 | 0.009 | - |
N-methyl-L-phenylalanine | wild-type, 37°C, pH not specified in the publication | Thermomicrobium roseum | |
| 1.5.3.1 | 0.0295 | - |
N-methyl-L-aspartate | wild-type, 37°C, pH not specified in the publication | Thermomicrobium roseum | |
| 1.5.3.1 | 0.0458 | - |
2-isopropyl-4-(methylaminomethyl)thiazole | wild-type, 37°C, pH not specified in the publication | Thermomicrobium roseum | |
| 1.5.3.1 | 0.053 | - |
sarcosine | wild-type, 37°C, pH not specified in the publication | Thermomicrobium roseum | |
| 1.5.3.1 | 0.0537 | - |
N-Methyl-L-leucine | wild-type, 37°C, pH not specified in the publication | Thermomicrobium roseum | |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.5.3.1 | 0.000015 | - |
N-methyl-D-proline | wild-type, 37°C, pH not specified in the publication | Thermomicrobium roseum | |
| 1.5.3.1 | 0.000033 | - |
N-methyl-L-tryptophan | wild-type, 37°C, pH not specified in the publication | Thermomicrobium roseum | |
| 1.5.3.1 | 0.00004 | - |
4-(ethylaminomethyl)pyridine | wild-type, 37°C, pH not specified in the publication | Thermomicrobium roseum | |
| 1.5.3.1 | 0.000051 | - |
N-methyl-L-phenylalanine | wild-type, 37°C, pH not specified in the publication | Thermomicrobium roseum | |
| 1.5.3.1 | 0.00012 | - |
N-methyl-L-aspartate | wild-type, 37°C, pH not specified in the publication | Thermomicrobium roseum | |
| 1.5.3.1 | 0.00021 | - |
N-Methyl-L-leucine | wild-type, 37°C, pH not specified in the publication | Thermomicrobium roseum | |
| 1.5.3.1 | 0.0003 | - |
L-proline | wild-type, 37°C, pH not specified in the publication | Thermomicrobium roseum | |
| 1.5.3.1 | 0.00033 | - |
2-isopropyl-4-(methylaminomethyl)thiazole | wild-type, 37°C, pH not specified in the publication | Thermomicrobium roseum | |
| 1.5.3.1 | 0.00087 | - |
sarcosine | wild-type, 37°C, pH not specified in the publication | Thermomicrobium roseum | |
| 1.5.3.1 | 0.016 | - |
N-Methyl-L-alanine | wild-type, 37°C, pH not specified in the publication | Thermomicrobium roseum | |
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