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Literature summary extracted from

  • Mohn, M.A.; Thaqi, B.; Fischer-Schrader, K.
    Isoform-specific NO synthesis by Arabidopsis thaliana nitrate reductase (2019), Plants (Basel), 8, 67 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.7.1.1 recombinant expression as two separate fragments in Escherichia coli, the N-terminal Nia1-Mo-heme fragment and a C-terminal fragment containing hinge 2 and the FAD-domain, residues 628-917 Arabidopsis thaliana
1.7.1.1 recombinant expression as two separate fragments in Escherichia coli, the N-terminal Nia2-Mo-heme fragment and a C-terminal fragment containing hinge 2 and the FAD-domain, residues 626-917 Arabidopsis thaliana
1.7.1.15 recombinant expression as two separate fragments in Escherichia coli, the N-terminal Nia1-Mo-heme fragment and a C-terminal fragment containing hinge 2 and the FAD-domain, residues 628-917 Arabidopsis thaliana
1.7.1.15 recombinant expression as two separate fragments in Escherichia coli, the N-terminal Nia2-Mo-heme fragment and a C-terminal fragment containing hinge 2 and the FAD-domain, residues 626-917 Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
1.7.1.1 additional information expression of the N-terminal fragment of NIA1, residues 1-627, i.e. NIA1-Mo-heme Arabidopsis thaliana
1.7.1.1 additional information expression of the N-terminal fragment of NIA2, residues 1-625, i.e. NIA2-Mo-heme Arabidopsis thaliana
1.7.1.15 additional information expression of the N-terminal fragment of NIA1, residues 1-627, i.e. NIA1-Mo-heme Arabidopsis thaliana
1.7.1.15 additional information expression of the N-terminal fragment of NIA2, residues 1-625, i.e. NIA2-Mo-heme Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.1.1 0.017
-
 nitrate N-terminal plus C-terminal fragment, pH 7, 22°C Arabidopsis thaliana
1.7.1.1 0.035
-
 nitrate N-terminal plus C-terminal fragment, pH 7, 22°C Arabidopsis thaliana
1.7.1.1 0.443
-
 nitrate N-terminal fragment, pH 7, 22°C Arabidopsis thaliana
1.7.1.1 2.12
-
 nitrate N-terminal fragment, pH 7, 22°C Arabidopsis thaliana
1.7.1.15 0.0103
-
 nitrite N-terminal plus C-terminal fragment, pH 7, 22°C Arabidopsis thaliana
1.7.1.15 0.0137
-
 nitrite N-terminal fragment, pH 7, 22°C Arabidopsis thaliana
1.7.1.15 0.0353
-
 nitrite N-terminal plus C-terminal fragment, pH 7.5, 22°C Arabidopsis thaliana
1.7.1.15 0.0355
-
 nitrite N-terminal fragment, pH 7.5, 22°C Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
1.7.1.1 Arabidopsis thaliana P11035 isoform Nia2
-
1.7.1.1 Arabidopsis thaliana P11832 isoform Nia1
-
1.7.1.15 Arabidopsis thaliana P11035 isoform Nia2, cf. EC 1.7.1.1
-
1.7.1.15 Arabidopsis thaliana P11832 isoform Nia1, cf. EC 1.7.1.1
-

Renatured (Commentary)

EC Number Renatured (Comment) Organism
1.7.1.1 recombinant expression of the N-terminal Nia1-Mo-heme fragment and a C-terminal fragment containing hinge 2 and the FAD-domain, residues 628-917 and mixing of the fragments to restore the electron transfer path Arabidopsis thaliana
1.7.1.1 recombinant expression of the N-terminal Nia2-Mo-heme fragment and a C-terminal fragment containing hinge 2 and the FAD-domain, residues 626-917 and mixing of the fragments to restore the electron transfer path Arabidopsis thaliana
1.7.1.15 recombinant expression of the N-terminal Nia1-Mo-heme fragment and a C-terminal fragment containing hinge 2 and the FAD-domain, residues 628-917 and mixing of the fragments to restore the electron transfer path Arabidopsis thaliana
1.7.1.15 recombinant expression of the N-terminal Nia2-Mo-heme fragment and a C-terminal fragment containing hinge 2 and the FAD-domain, residues 626-917 and mixing of the fragments to restore the electron transfer path Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.1.1 additional information enzyme additionally reduces nitrite, reaction of EC 1.7.1.15 Arabidopsis thaliana ?
-
-
1.7.1.1 reduced methyl viologen + nitrate
-
 Arabidopsis thaliana methyl viologen + nitrite
-
 ?
1.7.1.15 nitrite + reduced methyl viologen
-
 Arabidopsis thaliana ammonia + oxidized methyl viologen
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.7.1.1 NIA1
-
 Arabidopsis thaliana
1.7.1.1 Nia2
-
 Arabidopsis thaliana
1.7.1.15 NIA1
-
 Arabidopsis thaliana
1.7.1.15 Nia2
-
 Arabidopsis thaliana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.7.1.1 9.6
-
 nitrate N-terminal plus C-terminal fragment, pH 7, 22°C Arabidopsis thaliana
1.7.1.1 13.4
-
 nitrate N-terminal plus C-terminal fragment, pH 7, 22°C Arabidopsis thaliana
1.7.1.1 51
-
 nitrate N-terminal fragment, pH 7, 22°C Arabidopsis thaliana
1.7.1.1 69
-
 nitrate N-terminal fragment, pH 7, 22°C Arabidopsis thaliana
1.7.1.15 1.2
-
 nitrite N-terminal plus C-terminal fragment, pH 7, 22°C Arabidopsis thaliana
1.7.1.15 1.8
-
 nitrite N-terminal fragment, pH 7, 22°C Arabidopsis thaliana
1.7.1.15 19.8
-
 nitrite N-terminal fragment, pH 7.5, 22°C Arabidopsis thaliana
1.7.1.15 137.5
-
 nitrite N-terminal plus C-terminal fragment, pH 7.5, 22°C Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.7.1.1 7
-
-
 Arabidopsis thaliana
1.7.1.15 7
-
-
 Arabidopsis thaliana

General Information

EC Number General Information Comment Organism
1.7.1.1 physiological function isoform NIA1 is the more efficient nitrite reductase while isoform NIA2 exhibits higher nitrate reductase activity Arabidopsis thaliana
1.7.1.15 physiological function isoform NIA1 is the more efficient nitrite reductase while isoform NIA2 exhibits higher nitrate reductase activity Arabidopsis thaliana
1.7.1.15 physiological function isoform NIA1 is the more efficient nitrite reductase while isoform NIA2 exhibits higher nitrate reductase activity, reaction of EC 1.7.1.1 Arabidopsis thaliana

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.7.1.1 24
-
 nitrate N-terminal fragment, pH 7, 22°C Arabidopsis thaliana
1.7.1.1 155
-
 nitrate N-terminal fragment, pH 7, 22°C Arabidopsis thaliana