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Literature summary extracted from

  • Kermasha, S.; Khalyfa, A.; Marsot, P.; Alli, I.; Fournier, R.
    Biomass production, purification and characterization of chlorophyllase from alga (Phaeodactylum tricornutum) (1992), Biotechnol. Appl. Biochem., 15, 142-159.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.14 dithiothreitol in combination with Mg2+ dithiothreitol can activate chlorophyllase-catalyzed chlorophyll hydrolysis Phaeodactylum tricornutum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.14 diisopropyl fluorophosphate
-
 Phaeodactylum tricornutum
3.1.1.14 iodoacetamide
-
 Phaeodactylum tricornutum
3.1.1.14 NEM
-
 Phaeodactylum tricornutum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.14 thylakoid membrane
-
 Phaeodactylum tricornutum 42651
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.14 Mg2+ in combination with dithiothreitol Mg2+ can activate chlorophyllase-catalyzed chlorophyll hydrolysis Phaeodactylum tricornutum

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.14 Phaeodactylum tricornutum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.14
-
 Phaeodactylum tricornutum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.14 9.79
-
 enzyme isolated at the exponential growth phase Phaeodactylum tricornutum
3.1.1.14 134.4
-
 enzyme isolated at the stationary growth phase Phaeodactylum tricornutum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.14 8.5
-
-
 Phaeodactylum tricornutum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.14 7.5 8.8 pH 7.5: about 35% of maximal activity, pH 8.8: about 65% of maximal activity Phaeodactylum tricornutum