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Literature summary extracted from
- Purification and properties of S-formylglutathione hydrolase from human liver (1974), J. Biol. Chem., 249, 7664-7672.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.1.2.12 | stabilization by 2-mercaptoethanol or dithiothreitol | Homo sapiens |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.2.12 | 2,4,6-Trinitrobenzene sulfonate | - |
Homo sapiens |  |
| 3.1.2.12 | 5,5'-dithiobis(2-nitrobenzoate) | - |
Homo sapiens | |
| 3.1.2.12 | arsenite | - |
Homo sapiens | |
| 3.1.2.12 | ascorbate | - |
Homo sapiens | |
| 3.1.2.12 | CaCl2 | - |
Homo sapiens | |
| 3.1.2.12 | Co2+ | - |
Homo sapiens | |
| 3.1.2.12 | CuSO4 | - |
Homo sapiens | |
| 3.1.2.12 | F- | - |
Homo sapiens | |
| 3.1.2.12 | folate | - |
Homo sapiens | |
| 3.1.2.12 | glutathione | - |
Homo sapiens | |
| 3.1.2.12 | HgCl2 | - |
Homo sapiens | |
| 3.1.2.12 | iodoacetate | - |
Homo sapiens | |
| 3.1.2.12 | p-hydroxymercuribenzoate | - |
Homo sapiens | |
| 3.1.2.12 | Zn2+ | - |
Homo sapiens | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.2.12 | 0.12 | - |
S-acetylglutathione | - |
Homo sapiens | |
| 3.1.2.12 | 0.29 | - |
S-formylglutathione | - |
Homo sapiens | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.1.2.12 | cytosol | - |
Homo sapiens | 5829 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.2.12 | 30000 | - |
2 * 30000, SDS-PAGE | Homo sapiens |
| 3.1.2.12 | 52000 | - |
gel filtration | Homo sapiens |
| 3.1.2.12 | 58200 | - |
calculation from sedimentation and diffusion coefficients | Homo sapiens |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.2.12 | Homo sapiens | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.2.12 | - |
Homo sapiens |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.2.12 | liver | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.2.12 | 4050 | - |
- |
Homo sapiens |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.1.2.12 | -70°C | Homo sapiens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.2.12 | S-acetylglutathione + H2O | slight activity | Homo sapiens | glutathione + acetate | - |
ir | |
| 3.1.2.12 | S-formylglutathione + H2O | - |
Homo sapiens | glutathione + formate | - |
ir | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.2.12 | dimer | 2 * 30000, SDS-PAGE | Homo sapiens |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.2.12 | additional information | - |
activation energy 4.5 cal/mol | Homo sapiens |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.2.12 | 6.9 | 7.1 | - |
Homo sapiens |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.2.12 | 4.6 | 8.6 | stable for 30 min at 20°C | Homo sapiens |
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