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Literature summary extracted from

  • Kato, N.; Sakazawa, C.; Nishizawa, T.; Tani, Y.; Yamada, H.
    Purificaton and characterization of S-formylglutathione hydrolase from a methanol-utilizing yeast, Kloeckera sp. No. 2201 (1980), Biochim. Biophys. Acta, 611, 323-332.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.2.12 5,5'-dithiobis(2-nitrobenzoate)
-
 Kloeckera sp.
3.1.2.12 arsenite
-
 Kloeckera sp.
3.1.2.12 CuSO4
-
 Kloeckera sp.
3.1.2.12 FeSO4
-
 Kloeckera sp.
3.1.2.12 HgCl2
-
 Kloeckera sp.
3.1.2.12 iodoacetate
-
 Kloeckera sp.
3.1.2.12 N-ethylmaleimide
-
 Kloeckera sp.
3.1.2.12 p-chloromercuribenzoate
-
 Kloeckera sp.
3.1.2.12 p-hydroxymercuribenzoate
-
 Kloeckera sp.
3.1.2.12 Zn2+
-
 Kloeckera sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.2.12 0.077
-
 S-formylglutathione
-
 Kloeckera sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.2.12 31000
-
 2 * 31000 SDS-PAGE Kloeckera sp.
3.1.2.12 58000
-
 gel filtration Kloeckera sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.2.12 S-formylglutathione + H2O [Candida] boidinii
-
 ?
-
 ?
3.1.2.12 S-formylglutathione + H2O Kloeckera sp. involved in the oxidation of methanol by methylotrophic yeasts ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.2.12 Kloeckera sp.
-
 No.2201, grown in presence of methanol
-
3.1.2.12 Ogataea angusta
-
-
-
3.1.2.12 Ogataea trehalophila
-
-
-
3.1.2.12 Torulopsis pinus
-
-
-
3.1.2.12 [Candida] boidinii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.2.12
-
 Kloeckera sp.

Storage Stability

EC Number Storage Stability Organism
3.1.2.12 -20°C Kloeckera sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.2.12 S-formylglutathione + H2O
-
 [Candida] boidinii glutathione + formate
-
 ?
3.1.2.12 S-formylglutathione + H2O
-
 Ogataea angusta glutathione + formate
-
 ?
3.1.2.12 S-formylglutathione + H2O
-
 Kloeckera sp. glutathione + formate
-
 ?
3.1.2.12 S-formylglutathione + H2O
-
 Ogataea trehalophila glutathione + formate
-
 ?
3.1.2.12 S-formylglutathione + H2O
-
 Torulopsis pinus glutathione + formate
-
 ?
3.1.2.12 S-formylglutathione + H2O
-
 [Candida] boidinii ?
-
 ?
3.1.2.12 S-formylglutathione + H2O involved in the oxidation of methanol by methylotrophic yeasts Kloeckera sp. ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.2.12 dimer
-
 [Candida] boidinii
3.1.2.12 dimer 2 * 31000 SDS-PAGE Kloeckera sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.2.12 50
-
-
 Kloeckera sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.2.12 45
-
-
 Kloeckera sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.2.12 6.4 6.6
-
 Kloeckera sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.2.12 7.2 7.6 5°C, stable for 4 d Kloeckera sp.