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Literature summary extracted from

  • Becker, A.; Hurwitz, J.
    The enzymatic cleavage of phosphate termini from polynucleotides (1967), J. Biol. Chem., 242, 936-950.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.33 p-hydroxymercuribenzoate in absence of sulfhydryl compounds Escherichia coli
3.1.3.34 p-hydroxymercuribenzoate
-
 Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.33 CoCl2 divalent metal required, 30% the rate observed with MgCl2 Escherichia coli
3.1.3.33 MgCl2 divalent metal required Escherichia coli
3.1.3.33 MnCl2 divalent metal required, 60% the rate observed with MgCl2 Escherichia coli
3.1.3.33 additional information no cleavage in absence of divalent metal ion Escherichia coli
3.1.3.34 Co2+ can replace Mg2+ Escherichia coli
3.1.3.34 Mg2+ required for maximal activity Escherichia coli
3.1.3.34 additional information effect of divalent cations alone or in combination Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.33 5'-phosphyl-terminated DNA + H2O Escherichia coli
-
 ?
-
 ?
3.1.3.34 deoxynucleoside 3'-phosphate + H2O Escherichia coli
-
 deoxynucleoside + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.33 Escherichia coli
-
 B, induced by T4am82
-
3.1.3.34 Escherichia coli
-
 induced by bacteriophages /T4am82
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.33 induced by T4am82 Escherichia coli
3.1.3.34 infected with T4am82 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.33 2460
-
-
 Escherichia coli
3.1.3.34 380
-
-
 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
3.1.3.34 -15°C, 6 months, glycerol, less than 10% loss of activity Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.33 5'-phosphyl-terminated DNA + H2O
-
 Escherichia coli ?
-
 ?
3.1.3.34 deoxynucleoside 3'-phosphate + H2O
-
 Escherichia coli deoxynucleoside + phosphate
-
 ?
3.1.3.34 pTp + H2O
-
 Escherichia coli pT + phosphate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.33 6.5
-
-
 Escherichia coli
3.1.3.34 5.6 6 sodium succinate buffer Escherichia coli
3.1.3.34 5.8 6.2 Tris-maleate buffer Escherichia coli
3.1.3.34 6 6.4 sodium cacodylate buffer Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.33 5.5 6.8 50% of maximal activity at pH 5.5 and pH 6.8 Escherichia coli