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Literature summary extracted from

  • Peng, Z.; Verma, D.P.S.
    A rice HAL2-like gene encodes a Ca(2+)-sensitive 3 (2 ),5-diphosphonucleoside 3 (2 )-phosphohydrolase and complements yeast met22 and Escherichia coli cysQ mutations (1995), J. Biol. Chem., 270, 29105-29110.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.1.3.7 D141A less than 5% of the wild-type activity Oryza sativa
3.1.3.7 D141N less than 5% of the wild-type activity Oryza sativa
3.1.3.7 D144N less than 5% of the wild-type activity Oryza sativa
3.1.3.7 D292A less than 5% of the wild-type activity Oryza sativa
3.1.3.7 D292N less than 5% of the wild-type activity Oryza sativa
3.1.3.7 W291L less than 5% of the wild-type activity Oryza sativa

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.7 Ca2+
-
Oryza sativa
3.1.3.7 Li+
-
Oryza sativa
3.1.3.7 Na+
-
Oryza sativa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.7 0.1
-
adenosine 3'-phosphate 5'-phosphosulfate
-
Oryza sativa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.7 Mg2+ activates Oryza sativa
3.1.3.7 Mg2+ dependend on Oryza sativa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.7 44000
-
calculation from sequence of cDNA Oryza sativa
3.1.3.7 47000
-
x * 47000, SDS-PAGE Oryza sativa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.7 additional information Oryza sativa adenosine 3',5'-bisphosphate is not the primary substrate, the enzyme may be important for response to salt stress ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.7 Oryza sativa P0C5A3 GenBank U33283
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.7 adenosine 2',5'-bisphosphate + H2O
-
Oryza sativa adenosine 5'-phosphate + phosphate
-
?
3.1.3.7 adenosine 3',5'-bisphosphate + H2O
-
Oryza sativa adenosine 5'-phosphate + phosphate
-
?
3.1.3.7 adenosine 3'-phosphate 5'-phosphosulfate + H2O
-
Oryza sativa adenosine 5'-phosphosulfate + phosphate
-
?
3.1.3.7 additional information adenosine 3',5'-bisphosphate is not the primary substrate, the enzyme may be important for response to salt stress Oryza sativa ?
-
?

Subunits

EC Number Subunits Comment Organism
3.1.3.7 ? x * 47000, SDS-PAGE Oryza sativa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.7 8.5
-
-
Oryza sativa