Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Ogasawara, W.; Abe, N.; Hagio, T.; Okada, H.; Morikawa, Y.
    Purification and characterization of a dipeptidyl carboxypeptidase from Pseudomonas sp. WO24 (1997), Biosci. Biotechnol. Biochem., 61, 858-863.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.15.1 1,10-phenanthroline
-
 Pseudomonas sp.
3.4.15.1 CaCl2
-
 Pseudomonas sp.
3.4.15.1 CuCl2
-
 Pseudomonas sp.
3.4.15.1 EDTA
-
 Pseudomonas sp.
3.4.15.1 MgCl2
-
 Pseudomonas sp.
3.4.15.1 Mn2+ MnCl2 Pseudomonas sp.
3.4.15.1 NaCl 500 mM, 50% inhibition Pseudomonas sp.
3.4.15.1 PO43- 100 mM, 50% inhibition Pseudomonas sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.15.1 NaCl 500 mM, 50% inhibition Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.15.1 72000
-
 gel filtration Pseudomonas sp.
3.4.15.1 74000
-
 1 * 74000, SDS-PAGE Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
3.4.15.1 Pseudomonas sp.
-
 WO24
-
3.4.15.1 Pseudomonas sp. WO24
-
 WO24
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.15.1
-
 Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.15.1 780
-
-
 Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.15.1 Ala-Ala-Ala-Ala + H2O
-
 Pseudomonas sp. Ala-Ala + Ala-Ala
-
 ?
3.4.15.1 Ala-Ala-Ala-Ala + H2O
-
 Pseudomonas sp. WO24 Ala-Ala + Ala-Ala
-
 ?
3.4.15.1 angiotensin I + H2O
-
 Pseudomonas sp. angiotensin II + His-Leu
-
 ?
3.4.15.1 angiotensin I + H2O
-
 Pseudomonas sp. WO24 angiotensin II + His-Leu
-
 ?
3.4.15.1 benzoyl-Gly-Ala-Pro + H2O
-
 Pseudomonas sp. benzoyl-Gly + Ala-Pro
-
 ?
3.4.15.1 benzoyl-Gly-Ala-Pro + H2O
-
 Pseudomonas sp. WO24 benzoyl-Gly + Ala-Pro
-
 ?
3.4.15.1 benzoyl-Gly-His-Leu + H2O
-
 Pseudomonas sp. benzoyl-Gly + His-Leu
-
 ?
3.4.15.1 benzoyl-Gly-His-Leu + H2O
-
 Pseudomonas sp. WO24 benzoyl-Gly + His-Leu
-
 ?
3.4.15.1 benzyloxycarbonyl-(NO2)Phe-His-Leu + H2O
-
 Pseudomonas sp. benzyloxycarbonyl-(NO2)Phe + His-Leu
-
 ?
3.4.15.1 benzyloxycarbonyl-Phe-Tyr-Leu
-
 Pseudomonas sp. benzyloxycarbonyl-Phe + Tyr-Leu
-
 ?
3.4.15.1 bradykinin + H2O
-
 Pseudomonas sp. Phe-Arg + Ser-Pro + Arg-Pro-Pro-Gly-Phe hydrolyzes Phe-Arg + Ser-Pro from the C-terminus of bradykinin ?
3.4.15.1 bradykinin + H2O
-
 Pseudomonas sp. WO24 Phe-Arg + Ser-Pro + Arg-Pro-Pro-Gly-Phe hydrolyzes Phe-Arg + Ser-Pro from the C-terminus of bradykinin ?

Subunits

EC Number Subunits Comment Organism
3.4.15.1 monomer 1 * 74000, SDS-PAGE Pseudomonas sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.15.1 50
-
-
 Pseudomonas sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.15.1 37
-
 stable for at least 30 min Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.15.1 6.5 7
-
 Pseudomonas sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.15.1 5.5 12 no activity below pH 5.5 and above pH 12.0 Pseudomonas sp.

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.15.1 100
-
 PO43-
-
 Pseudomonas sp.
3.4.15.1 500
-
 NaCl
-
 Pseudomonas sp.