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Literature summary extracted from

  • Patel, R.N.; Mazumdar, S.; Ornston, L.N.
    beta-Ketoadipate enol-lactone hydrolases I and II from Acinetobacter calcoaceticus (1975), J. Biol. Chem., 250, 6567-6577.
    View publication on PubMed

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.24 12000
-
2 * 12000, SDS-Page Acinetobacter calcoaceticus
3.1.1.24 25000
-
ELH I and ELH II, gel filtration Acinetobacter calcoaceticus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.24 (5-oxo-4,5-dihydrofuran-2-yl)acetic acid + H2O Acinetobacter calcoaceticus the enzyme acts on the product of EC 4.1.1.44 4-carboxymuconolactone decarboxylase ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.24 Acinetobacter calcoaceticus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.24 ELH I and II, 290fold, and 550fold Acinetobacter calcoaceticus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.24 500
-
ELH II Acinetobacter calcoaceticus
3.1.1.24 580
-
ELH I Acinetobacter calcoaceticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.24 (5-oxo-4,5-dihydrofuran-2-yl)acetic acid + H2O 3-oxoadipate enol-lactone Acinetobacter calcoaceticus 3-oxoadipate
-
?
3.1.1.24 (5-oxo-4,5-dihydrofuran-2-yl)acetic acid + H2O the enzyme acts on the product of EC 4.1.1.44 4-carboxymuconolactone decarboxylase Acinetobacter calcoaceticus ?
-
?

Subunits

EC Number Subunits Comment Organism
3.1.1.24 dimer 2 * 12000, SDS-Page Acinetobacter calcoaceticus
3.1.1.24 More primarily as dimers, two further bands appear, the protein may associate to form higher oligomers Acinetobacter calcoaceticus

Synonyms

EC Number Synonyms Comment Organism
3.1.1.24 beta-ketoadipate enol-lactone hydrolase form I and II Acinetobacter calcoaceticus
3.1.1.24 ELH I protocatechuate-induced Acinetobacter calcoaceticus
3.1.1.24 ELH II cis, cis-muconate-induced Acinetobacter calcoaceticus