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Literature summary extracted from

  • Fujikura, Y.; Baisted, D.
    Purification and characterization of a basic lysophospholipase in germinating barley (1985), Arch. Biochem. Biophys., 243, 570-578.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.5 2-mercaptoethanol minimizes loss of activity in presence of sulfhydryl reagent Hordeum vulgare

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.5 Ca2+ modest Hordeum vulgare
3.1.1.5 Cu2+ little effect Hordeum vulgare
3.1.1.5 Hg2+ little effect Hordeum vulgare
3.1.1.5 iodoacetamide
-
 Hordeum vulgare
3.1.1.5 NEM little effect Hordeum vulgare
3.1.1.5 PCMB
-
 Hordeum vulgare
3.1.1.5 PMSF little effect Hordeum vulgare

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.5 0.03
-
 2-lysophosphatidylcholine
-
 Hordeum vulgare

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.5 40000
-
 x * 40000, PLP I, SDS-PAGE Hordeum vulgare

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.5 additional information Hordeum vulgare the function of the enzyme is directed to the disposal of the starch inclusion lipids during their release from the starch matrix during germination ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.5 Hordeum vulgare
-
 var. Advance, at least 3 enzymatic forms: PLP I, PLP II and PLP II'
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.1.5 side-chain modification glycoprotein Hordeum vulgare
3.1.1.5 side-chain modification 10-12% carbohydrate Hordeum vulgare

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.5
-
 Hordeum vulgare

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.5 seed
-
 Hordeum vulgare
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.5 51
-
-
 Hordeum vulgare

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.5 1-palmitoyl-sn-glycero-3-phosphocholine + H2O
-
 Hordeum vulgare palmitic acid + glycero-3-phosphocholine
-
 ?
3.1.1.5 2-lysophosphatidylcholine + H2O
-
 Hordeum vulgare glycerophosphorylcholine + a carboxylate
-
 ?
3.1.1.5 additional information the function of the enzyme is directed to the disposal of the starch inclusion lipids during their release from the starch matrix during germination Hordeum vulgare ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.5 ? x * 40000, PLP I, SDS-PAGE Hordeum vulgare

Synonyms

EC Number Synonyms Comment Organism
3.1.1.5 LPL
-
 Hordeum vulgare

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.5 8
-
 Tris buffer Hordeum vulgare

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.5 5 9 pH 5.0: about 40% of maximal activity, pH 9.0: about 50% of maximal activity Hordeum vulgare