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Literature summary extracted from
- Alkaline phosphatase from Paramecium cilia and cell bodies: purification and characterization (1990), Biochim. Biophys. Acta, 1037, 233-239.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.1.3.1 | repeated freezing and thawing in the presence of mercaptoethanol has no effect | Paramecium tetraurelia |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.3.1 | Ca2+ | in presence of 0.005 mM Mg2+, Ca2+ acts as an uncompetitive inhibitor, at 0.1 mM Mg2+, Ca2+ inhibits non-competitively | Paramecium tetraurelia |  |
| 3.1.3.1 | diphosphate | - |
Paramecium tetraurelia | |
| 3.1.3.1 | F- | - |
Paramecium tetraurelia | |
| 3.1.3.1 | molybdate | - |
Paramecium tetraurelia | |
| 3.1.3.1 | NEM | - |
Paramecium tetraurelia | |
| 3.1.3.1 | p-hydroxymercuribenzoate | - |
Paramecium tetraurelia | |
| 3.1.3.1 | phosphate | - |
Paramecium tetraurelia | |
| 3.1.3.1 | SH-group blocking agents | - |
Paramecium tetraurelia | |
| 3.1.3.1 | vanadate | - |
Paramecium tetraurelia | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.3.1 | 0.081 | - |
p-nitrophenyl phosphate | - |
Paramecium tetraurelia | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.1.3.1 | cilium | - |
Paramecium tetraurelia | 5929 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.3.1 | Mg2+ | non-essential mixed-type activator, half-maximal effect at 0.007 mM | Paramecium tetraurelia | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.1 | 66000 | - |
gel filtration | Paramecium tetraurelia |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.3.1 | Paramecium tetraurelia | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.3.1 | - |
Paramecium tetraurelia |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.1 | additional information | - |
- |
Paramecium tetraurelia |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.1.3.1 | -70°C, stable for at least 6 months | Paramecium tetraurelia |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.3.1 | additional information | enzyme with limited substrate specificity, no dephosphorylation of ATP, PEP, glucose 6-phosphate, phosphoinositol 4',5'-bisphosphate, phosphoproteins | Paramecium tetraurelia | ? | - |
? | |
| 3.1.3.1 | p-nitrophenyl phosphate + H2O | - |
Paramecium tetraurelia | p-nitrophenol + phosphate | - |
? | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.1 | 8 | - |
- |
Paramecium tetraurelia |
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