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Literature summary extracted from

  • Kobori, H.; Taga, N.
    Extracellular alkaline phosphatase from marine bacteria: purification and properties of extracellular phosphatase from a marine Pseudomonas sp. (1980), Can. J. Microbiol., 26, 833-838.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.3.1 anions required for maximal activity in the order of decreasing effectiveness: Cl-, Br-, NO3-, ClO4-, SCN- Pseudomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.1 Cys
-
 Pseudomonas sp.
3.1.3.1 EDTA
-
 Homo sapiens
3.1.3.1 EDTA inhibition is reversed by divalent cations Pseudomonas sp.
3.1.3.1 Hg2+ 1 mM, 82% loss of activity Pseudomonas sp.
3.1.3.1 KCN
-
 Pseudomonas sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.1 extracellular
-
 Pseudomonas sp.
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.1 100000
-
 gel filtration Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.1 Homo sapiens
-
-
-
3.1.3.1 Pseudomonas sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.1
-
 Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.1 192
-
-
 Pseudomonas sp.

Storage Stability

EC Number Storage Stability Organism
3.1.3.1 5°C, pH 8.0, Tris-HCl buffer, in seawater, 10 days, 50% inactivation Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.1 2-phosphoglycerate + H2O
-
 Homo sapiens glycerol + phosphate
-
 ?
3.1.3.1 2-phosphoglycerate + H2O
-
 Pseudomonas sp. glycerol + phosphate
-
 ?
3.1.3.1 ADP + H2O
-
 Homo sapiens ?
-
 ?
3.1.3.1 ADP + H2O
-
 Pseudomonas sp. ?
-
 ?
3.1.3.1 AMP + H2O
-
 Homo sapiens adenosine + phosphate
-
 ?
3.1.3.1 AMP + H2O
-
 Pseudomonas sp. adenosine + phosphate
-
 ?
3.1.3.1 ATP + H2O
-
 Homo sapiens ?
-
 ?
3.1.3.1 ATP + H2O
-
 Pseudomonas sp. ?
-
 ?
3.1.3.1 diphosphate + H2O no activity Pseudomonas sp. 2 phosphate
-
 ?
3.1.3.1 glucose 1-phosphate + H2O
-
 Homo sapiens glucose + phosphate
-
 ?
3.1.3.1 glucose 1-phosphate + H2O
-
 Pseudomonas sp. glucose + phosphate
-
 ?
3.1.3.1 Glucose 6-phosphate + H2O
-
 Pseudomonas sp. Glucose + phosphate
-
 ?
3.1.3.1 GMP + H2O
-
 Pseudomonas sp. guanosine + phosphate
-
 ?
3.1.3.1 GTP + H2O
-
 Pseudomonas sp. ?
-
 ?
3.1.3.1 p-nitrophenyl phosphate + H2O
-
 Homo sapiens p-nitrophenol + phosphate
-
 ?
3.1.3.1 p-nitrophenyl phosphate + H2O
-
 Pseudomonas sp. p-nitrophenol + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.1 monomer 1 * 100000, SDS-PAGE Pseudomonas sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.1 50
-
-
 Homo sapiens
3.1.3.1 50
-
 10 min, partial inactivation Pseudomonas sp.
3.1.3.1 75
-
 10 min, complete inactivation Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.1 10.5
-
-
 Homo sapiens