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Literature summary extracted from

  • Gratecos, D.; Detwiler, T.C.; Hurd, S.; Fischer, E.H.
    Rabbit muscle phosphorylase phosphatase. 1. Purification and chemical properties (1977), Biochemistry, 16, 4812-4817.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.3.17 2-mercaptoethanol maximal activation at 50 mM, absolute requirement for SH compounds Oryctolagus cuniculus
3.1.3.17 ascorbic acid maximal activation at 1 mM Oryctolagus cuniculus
3.1.3.17 dithiothreitol maximal activation at 1 mM, absolute requirement for SH compounds Oryctolagus cuniculus

General Stability

EC Number General Stability Organism
3.1.3.17 complete inactivation after treatment with 0.3 M perchloric acid or 10% trichloroacetic acid Oryctolagus cuniculus
3.1.3.17 complete inactivation after treatment with 1% dodecyl sulfate or 0.05% cetyltrimethylammonium bromide or 1 M guanidine-HCl after 15 min Oryctolagus cuniculus
3.1.3.17 complete inactivation after treatment with 10% w/w pronase at 30°C for 3 h Oryctolagus cuniculus
3.1.3.17 resistant to trypsin and chymotrypsin and to 8 M urea Oryctolagus cuniculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.17 2-hydroxyethyl disulfide
-
 Oryctolagus cuniculus
3.1.3.17 5,5'-dithiobis(2-nitrobenzoic acid)
-
 Oryctolagus cuniculus
3.1.3.17 Ca2+ 10 mM, 20% inhibition Oryctolagus cuniculus
3.1.3.17 cystamine
-
 Oryctolagus cuniculus
3.1.3.17 cystine
-
 Oryctolagus cuniculus
3.1.3.17 D-Pantethine
-
 Oryctolagus cuniculus
3.1.3.17 Disulfides irreversible inactivation Oryctolagus cuniculus
3.1.3.17 GSSG
-
 Oryctolagus cuniculus
3.1.3.17 iodoacetamide 50 mM, 30% inhibition after 1 h at 30°C Oryctolagus cuniculus
3.1.3.17 iodoacetic acid 50 mM, 30% inhibition after 1 h at 30°C Oryctolagus cuniculus
3.1.3.17 Mg2+ 10 mM, 20% inhibition Oryctolagus cuniculus
3.1.3.17 Mn2+ 3 mM, 64% inhibition Oryctolagus cuniculus
3.1.3.17 NaCl 0.1-0.3 M, 40-96% inhibition Oryctolagus cuniculus
3.1.3.17 poly(L-lysine) 0.001 mM, strong Oryctolagus cuniculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.17 Mn2+ inactive in absence of Mn2+ Oryctolagus cuniculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.17 33000
-
 gel filtration Oryctolagus cuniculus

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.1.3.17 deoxycholate stable to 0.5% deoxycholate, 24 h Oryctolagus cuniculus
3.1.3.17 Ethanol stable to 25% ethanol at -5°C, 3 h Oryctolagus cuniculus
3.1.3.17 Triton X-100 stable to 0.5% Triton X-100, 24 h Oryctolagus cuniculus
3.1.3.17 Tween stable to 0.1% Tween 20, 40 or 80, 24 h Oryctolagus cuniculus

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.17 Oryctolagus cuniculus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.3.17 no modification the enzyme is free of bound phosphate, carbohydrate, lipids and nucleic acids Oryctolagus cuniculus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.17
-
 Oryctolagus cuniculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.17 muscle
-
 Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.17 1
-
-
 Oryctolagus cuniculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.17 phosphorylase a + H2O
-
 Oryctolagus cuniculus phosphorylase b + phosphate
-
 ?
3.1.3.17 troponin I + H2O
-
 Oryctolagus cuniculus phosphate + ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.17 60
-
 15 min, 50% loss of activity Oryctolagus cuniculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.17 7.8
-
-
 Oryctolagus cuniculus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.17 6.5 8.7 about 50% of maximal activity at pH 6.5 and at pH 8.7 Oryctolagus cuniculus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.3.17 5
-
 30 min, 90% loss of activity Oryctolagus cuniculus