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Literature summary extracted from

  • Gibson, D.M.; Ullah, A.H.J.
    Purification and characterization of phytase from cotyledons of germinating soybean seeds (1988), Arch. Biochem. Biophys., 260, 503-513.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.26 F- NaF Glycine max
3.1.3.26 Fe2+
-
Glycine max
3.1.3.26 Hg2+
-
Glycine max
3.1.3.26 NaH2PO4
-
Glycine max
3.1.3.26 oxalate
-
Glycine max
3.1.3.26 tentoxin weak Glycine max
3.1.3.26 vanadate
-
Glycine max
3.1.3.26 Zn2+
-
Glycine max

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.26 Ca2+ 1 mM, stimulates 40% over control Hordeum vulgare
3.1.3.26 Mg2+ 0.2-5 mM, slight stimulation Glycine max
3.1.3.26 Mn2+ activates Glycine max

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.26 50000
-
gel filtration Glycine max

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.26 Glycine max
-
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
3.1.3.26 Hordeum vulgare
-
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.26
-
Glycine max

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.26 cotyledon
-
Glycine max
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.26 additional information
-
-
Glycine max

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.26 ATP + H2O
-
Glycine max ?
-
?
3.1.3.26 bis(p-nitrophenyl)phosphate + H2O
-
Glycine max ?
-
?
3.1.3.26 diphosphate + H2O
-
Glycine max 2 phosphate
-
?
3.1.3.26 glycerol 2-phosphate + H2O
-
Glycine max glycerol + phosphate
-
?
3.1.3.26 myo-inositol hexakisphosphate + H2O
-
Hordeum vulgare myo-inositol pentakisphosphate + phosphate
-
?
3.1.3.26 myo-inositol hexakisphosphate + H2O
-
Glycine max myo-inositol pentakisphosphate + phosphate
-
?
3.1.3.26 p-nitrophenyl phosphate + H2O
-
Glycine max p-nitrophenol + phosphate
-
?
3.1.3.26 phenyl phosphate + H2O
-
Glycine max phenol + phosphate
-
?

Subunits

EC Number Subunits Comment Organism
3.1.3.26 monomer 1 * 59000-60000, SDS-PAGE Glycine max

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.26 55
-
-
Glycine max

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.26 40 55 10 min, no loss of activity Glycine max
3.1.3.26 70
-
10 min, 91% loss of activity Glycine max

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.26 4.5 4.8
-
Glycine max

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.26 3.5 5 pH 3.5: about 40% of maximal activity, pH 5.5: about 75% of maximal activity Glycine max