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Literature summary extracted from

  • El-Maghrabi, M.R.; Correia, J.J.; Heil, P.J.; Pate, T.M.; Cobb, C.E.; Pilkis, S.J.
    Tissue distribution, immunoreactivity, and physical properties of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (1986), Proc. Natl. Acad. Sci. USA, 83, 5005-5009.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.105 iodoacetamide activation, kidney and liver, not heart or testis enzyme Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.105 additional information phosphorylation by cAMP-dependent protein kinase causes inactivation of liver enzyme Bos taurus
2.7.1.105 additional information phosphorylation by cAMP-dependent protein kinase causes inactivation of liver enzyme; phosphorylation by cAMP-dependent protein kinase causes inactivation of liver enzyme, not of kidney, testis and heart enzyme Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.105 Mg2+
-
 Rattus norvegicus
2.7.1.105 Mg2+
-
 Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.105 55000
-
 2 * 55000, SDS-PAGE Rattus norvegicus
2.7.1.105 107000 109000 equilibrium sedimentation, sedimentation velocity analysis Rattus norvegicus
3.1.3.46 107000
-
 equilibrium sedimentation Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.105 ATP + beta-D-fructose 6-phosphate Bos taurus
-
 ADP + beta-D-fructose 2,6-bisphosphate
-
 ?
2.7.1.105 ATP + beta-D-fructose 6-phosphate Rattus norvegicus enzyme plays an important role in the regulation of hepatic carbohydrate metabolism ADP + beta-D-fructose 2,6-bisphosphate
-
 ?
3.1.3.46 fructose 2,6-bisphosphate + H2O Rattus norvegicus
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.105 Bos taurus
-
-
-
2.7.1.105 Canis lupus familiaris
-
-
-
2.7.1.105 Rattus norvegicus
-
-
-
3.1.3.46 Rattus norvegicus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.1.105 phosphoprotein phosphorylation by cAMP-dependent protein kinase causes inactivation of liver enzyme, not of kidney, testis and heart enzyme Rattus norvegicus
2.7.1.105 phosphoprotein phosphorylation by cAMP-dependent protein kinase causes inactivation of liver enzyme Rattus norvegicus
2.7.1.105 phosphoprotein phosphorylation by cAMP-dependent protein kinase causes inactivation of liver enzyme Bos taurus

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.105
-
 Rattus norvegicus

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.3.46 beta-D-fructose 2,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate bifunctional enzyme: 6-phosphofructo-2-kinase-fructose-2,6-bisphosphatase Rattus norvegicus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.105 heart
-
 Rattus norvegicus
-
2.7.1.105 heart
-
 Bos taurus
-
2.7.1.105 kidney
-
 Rattus norvegicus
-
2.7.1.105 liver
-
 Rattus norvegicus
-
2.7.1.105 liver
-
 Bos taurus
-
2.7.1.105 liver
-
 Canis lupus familiaris
-
2.7.1.105 lung
-
 Rattus norvegicus
-
2.7.1.105 additional information tissue distribution, in extrahepatic tissue only 10% or less of activity in liver Rattus norvegicus
-
2.7.1.105 skeletal muscle
-
 Rattus norvegicus
-
2.7.1.105 testis
-
 Rattus norvegicus
-
3.1.3.46 adipose tissue
-
 Rattus norvegicus
-
3.1.3.46 brain
-
 Rattus norvegicus
-
3.1.3.46 kidney
-
 Rattus norvegicus
-
3.1.3.46 liver
-
 Rattus norvegicus
-
3.1.3.46 lung
-
 Rattus norvegicus
-
3.1.3.46 muscle skeletal Rattus norvegicus
-
3.1.3.46 spleen
-
 Rattus norvegicus
-
3.1.3.46 testis
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.105 ATP + beta-D-fructose 6-phosphate
-
 Rattus norvegicus ADP + beta-D-fructose 2,6-bisphosphate
-
 ?
2.7.1.105 ATP + beta-D-fructose 6-phosphate
-
 Bos taurus ADP + beta-D-fructose 2,6-bisphosphate
-
 ?
2.7.1.105 ATP + beta-D-fructose 6-phosphate
-
 Canis lupus familiaris ADP + beta-D-fructose 2,6-bisphosphate
-
 ?
2.7.1.105 ATP + beta-D-fructose 6-phosphate enzyme plays an important role in the regulation of hepatic carbohydrate metabolism Rattus norvegicus ADP + beta-D-fructose 2,6-bisphosphate
-
 ?
2.7.1.105 additional information also catalyses the degradation of fructose 2,6-bisphosphate (EC 3.1.3.46) Rattus norvegicus ?
-
 ?
2.7.1.105 additional information also catalyses the degradation of fructose 2,6-bisphosphate (EC 3.1.3.46) Bos taurus ?
-
 ?
2.7.1.105 additional information also catalyses the degradation of fructose 2,6-bisphosphate (EC 3.1.3.46) Canis lupus familiaris ?
-
 ?
2.7.1.105 additional information allosteric bifunctional enzyme, not heart Rattus norvegicus ?
-
 ?
3.1.3.46 fructose 2,6-bisphosphate + H2O
-
 Rattus norvegicus fructose 6-phosphate + phosphate
-
 ?
3.1.3.46 fructose 2,6-bisphosphate + H2O
-
 Rattus norvegicus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.105 dimer
-
 Bos taurus
2.7.1.105 dimer
-
 Canis lupus familiaris
2.7.1.105 dimer 2 * 55000, SDS-PAGE Rattus norvegicus
3.1.3.46 dimer 2 * 50000-55000, SDS-PAGE Rattus norvegicus