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Literature summary extracted from
- Characterization of the pcp gene of Pseudomonas fluorescens and of its product, pyrrolidone carboxyl peptidase (Pcp) (1994), J. Bacteriol., 176, 2569-2576.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.4.19.3 | - |
Pseudomonas fluorescens |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.19.3 | 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide | - |
Pseudomonas fluorescens |  |
| 3.4.19.3 | diethyl dicarbonate | - |
Pseudomonas fluorescens | |
| 3.4.19.3 | iodoacetamide | - |
Pseudomonas fluorescens | |
| 3.4.19.3 | NEM | - |
Pseudomonas fluorescens | |
| 3.4.19.3 | PCMB | - |
Pseudomonas fluorescens | |
| 3.4.19.3 | PMSF | - |
Pseudomonas fluorescens | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.19.3 | 0.21 | - |
L-pyroglutamyl-beta-naphthylamide | - |
Pseudomonas fluorescens | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.19.3 | 22441 | - |
2 * 22441, calculation from nucleotide sequence | Pseudomonas fluorescens |
| 3.4.19.3 | 23000 | - |
2 * 23000, SDS-PAGE | Pseudomonas fluorescens |
| 3.4.19.3 | 41000 | - |
gel filtration | Pseudomonas fluorescens |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.19.3 | Pseudomonas fluorescens | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.19.3 | - |
Pseudomonas fluorescens |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.4.19.3 | 73.7 | - |
- |
Pseudomonas fluorescens |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.4.19.3 | 4°C, -20°C or -70°C, 1 month, 90% loss of activity | Pseudomonas fluorescens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.19.3 | pyroglutamyl-beta-naphthylamide + H2O | - |
Pseudomonas fluorescens | pyroglutamate + beta-naphthylamine | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.19.3 | dimer | 2 * 23000, SDS-PAGE | Pseudomonas fluorescens |
| 3.4.19.3 | dimer | 2 * 22441, calculation from nucleotide sequence | Pseudomonas fluorescens |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.19.3 | 30 | - |
- |
Pseudomonas fluorescens |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.19.3 | 37 | - |
1 h, rapid inactivation | Pseudomonas fluorescens |
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