EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.4.19.6 | DMSO | activity using substrates containing 2% v/v DMSO is 5% higher than that using substrate containing no DSMO | Bos taurus |
EC Number | General Stability | Organism |
---|---|---|
3.4.19.3 | addition of 1% bovine serum albumin to the purified enzyme improves stability with 88% of the activity remaining after 4 days at 4°C and 93% of the activity remaining after 21 days at -80°C | Bos taurus |
3.4.19.3 | enzyme solubilized by sodium deoxycholate and Triton X-100, -20°C or 4°C, 24 h, more than 50% loss of activity | Bos taurus |
3.4.19.3 | trypsin solubilized enzyme, no loss of activity after 14 days at 4°C, 20% loss of activity after 14 days at -20°C | Bos taurus |
3.4.19.6 | 1% w/v bovine serum albumin greatly improves stability with 88% of the activity remaining after 4 days at 4°C and 93% of the activity remaining after 21 days at -80°C | Bos taurus |
3.4.19.6 | addition of 20% v/v glyceriol affords no protection of the enzyme stored at 4°C and only moderate protection at -20°C and -80°C | Bos taurus |
3.4.19.6 | trypsin is the most effective protease, above 0.001 mg/ml, the solubilized enzyme st stable for 14 days at 4°C, approximately 80% of the activity remains after 14 days at -20°C | Bos taurus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.19.3 | 1,10-phenanthroline | - |
Bos taurus | |
3.4.19.3 | 1,7-phenanthroline | - |
Bos taurus | |
3.4.19.3 | 4,7-phenanthroline | - |
Bos taurus | |
3.4.19.3 | 4-(2-aminoethyl)-benzenesulfonylfluoride | - |
Bos taurus | |
3.4.19.3 | 8-hydroxyquinoline | - |
Bos taurus | |
3.4.19.3 | Cd2+ | - |
Bacillus amyloliquefaciens | |
3.4.19.3 | Cd2+ | CdSO4 | Bos taurus | |
3.4.19.3 | Dithiobisnitrobenzoate | - |
Bos taurus | |
3.4.19.3 | dithiothreitol | - |
Bos taurus | |
3.4.19.3 | EGTA | - |
Bos taurus | |
3.4.19.3 | Hg2+ | - |
Bacillus amyloliquefaciens | |
3.4.19.3 | Hg2+ | HgSO4 | Bos taurus | |
3.4.19.3 | luteinizing hormone releasing hormone 1-5 | - |
Bos taurus | |
3.4.19.3 | thioglycolic acid | - |
Bos taurus | |
3.4.19.3 | Zn2+ | ZnSO4 | Bos taurus | |
3.4.19.6 | 1,7-phenanthroline | 10 mM, 67% inhibition | Bos taurus | |
3.4.19.6 | 4,7-phenanthroline | 10 mM, 56% inhibition | Bos taurus | |
3.4.19.6 | 4-(2-aminoethyl)-benzenesulfonylfluoride | 66% inhibition at 1 mM, 97% inhibition at 10 mM | Bos taurus | |
3.4.19.6 | 8-hydroxyquinoline | 10 mM, 68% inhibition | Bos taurus | |
3.4.19.6 | Cd2+ | 50% inhibition at 0.1 mM CsSO4, 98% inhibition at 1 mM | Bos taurus | |
3.4.19.6 | CDTA | 10 mM, 10% inhibition | Bos taurus | |
3.4.19.6 | CuSO4 | 0.1 mM | Bos taurus | |
3.4.19.6 | EDTA | 10 mM, 29% inhibition | Bos taurus | |
3.4.19.6 | Hg2+ | 1 mM HgSO4, 76% inhibition | Bos taurus | |
3.4.19.6 | Luteinizing hormone releasing hormone | - |
Bos taurus | |
3.4.19.6 | MgSO4 | 0.1 mM | Bos taurus | |
3.4.19.6 | NEM | 10 mM, 22% inhibition | Bos taurus | |
3.4.19.6 | puromycin | 1 mM, 17% inhibition | Bos taurus | |
3.4.19.6 | pyroglutamyl-7-amido-4-methylcoumarin | - |
Bos taurus | |
3.4.19.6 | pyroglutamyl-His | - |
Bos taurus | |
3.4.19.6 | Pyroglutamyl-His-Gly | - |
Bos taurus | |
3.4.19.6 | pyroglutamyl-His-Gly-NH2 | - |
Bos taurus | |
3.4.19.6 | pyroglutamyl-His-Pro | - |
Bos taurus | |
3.4.19.6 | pyroglutamyl-His-Pro-7-amido-4-methylcoumarin | - |
Bos taurus | |
3.4.19.6 | pyroglutamyl-His-Pro-Gly | - |
Bos taurus | |
3.4.19.6 | pyroglutamyl-His-Pro-Gly-NH2 | - |
Bos taurus | |
3.4.19.6 | pyroglutamyl-His-Pro-NH2 | - |
Bos taurus | |
3.4.19.6 | pyroglutamyl-His-Trp-Ser-Tyr-Gly | - |
Bos taurus | |
3.4.19.6 | pyroglutamyl-His-Trp-Ser-Tyr-Gly-Leu | - |
Bos taurus | |
3.4.19.6 | pyroglutamyl-His-Trp-Ser-Tyr-Gly-Leu-Arg | - |
Bos taurus | |
3.4.19.6 | pyroglutamyl-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly | - |
Bos taurus | |
3.4.19.6 | pyroglutamyl-Phe-Pro-NH2 | - |
Bos taurus | |
3.4.19.6 | ZnSO4 | 1 mM ZnSO4, 61% inhibition | Bos taurus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.19.3 | 0.00253 | - |
pyroglutamyl-His-Pro-4-methylcoumarin 7-amide | - |
Bos taurus | |
3.4.19.3 | 0.4198 | - |
pyroglutamyl-His-Pro-4-methylcoumarin 7-amide | - |
Bos taurus | |
3.4.19.6 | 0.00253 | - |
pyroglutamyl-His-Pro-7-amido-4-methylcoumarin | 37°C | Bos taurus | |
3.4.19.6 | 0.419 | - |
pyroglutamyl-7-amido-4-methylcoumarin | 37°C | Bos taurus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.4.19.3 | membrane | - |
Bos taurus | 16020 | - |
3.4.19.6 | membrane | - |
Bos taurus | 16020 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.4.19.3 | CoSO4 | activates | Bos taurus | |
3.4.19.3 | additional information | metallopeptidase | Bos taurus | |
3.4.19.6 | CoSO4 | 161% enhancement of activity at 0.1 mM and 559% enhancement at 1 mM. 1 mM, 587% reactivation of the enzyme inactivated by EDTA | Bos taurus | |
3.4.19.6 | MnSO4 | 0.1 mM, 56% reactivation of the enzyme inactivated by EDTA | Bos taurus | |
3.4.19.6 | NiSO4 | 1 mM, 185% reactivation of the enzyme inactivated by EDTA | Bos taurus | |
3.4.19.6 | ZnSO4 | 0.1 mM, 108% reactivation of the enzyme inactivated by EDTA | Bos taurus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.4.19.3 | 214000 | - |
gel filtration | Bos taurus |
3.4.19.6 | 214000 | - |
gel filtration | Bos taurus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.19.3 | Bacillus amyloliquefaciens | - |
- |
- |
3.4.19.3 | Bacillus subtilis | - |
- |
- |
3.4.19.3 | Bos taurus | - |
- |
- |
3.4.19.6 | Bos taurus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.19.3 | - |
Bos taurus |
3.4.19.6 | - |
Bos taurus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.4.19.3 | brain | - |
Bos taurus | - |
3.4.19.6 | brain | - |
Bos taurus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.4.19.3 | 0.0208 | - |
- |
Bos taurus |
3.4.19.6 | 0.0209 | - |
- |
Bos taurus |
EC Number | Storage Stability | Organism |
---|---|---|
3.4.19.3 | -20°C or 4°C, 24 h, more than 50% loss of activity of the enzyme solubilized by sodium deoxycholate and Triton X-100 | Bos taurus |
3.4.19.3 | 4°C, 72 h, 10% loss of activity of the enzyme solubilized in CHAPS | Bos taurus |
3.4.19.3 | 4°C, 72 h, 60% loss of activity of the enzyme solubilized in CHAPS | Bos taurus |
3.4.19.6 | 4°C or -20°C, 24 h, decrease of more than 50% of activity of the enzyme solubilized by sodium deoxycholate or Triton X-100, the CHAPS solubilized enzyme is slightly more stable with approximately 90% and 40% of the activity remaining after 72 h at 4°C and -20°C | Bos taurus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.19.3 | L-pyroglutamyl-His-Pro-4-methylcoumarin 7-amide + H2O | - |
Bos taurus | L-pyroglutamate + His-Pro-4-methylcoumarin 7-amide | - |
? | |
3.4.19.3 | pyroglutamyl-4-methylcoumarin 7-amide + H2O | - |
Bos taurus | pyroglutamate + 7-amino-4-methylcoumarin | - |
? | |
3.4.19.3 | pyroglutamyl-His-Gly + H2O | - |
Bos taurus | pyroglutamate + His-Gly | - |
? | |
3.4.19.3 | pyroglutamyl-His-Gly-NH2 + H2O | - |
Bos taurus | pyroglutamate + His-Gly-NH2 | - |
? | |
3.4.19.3 | pyroglutamyl-His-Pro-Gly + H2O | - |
Bos taurus | pyroglutamate + His-Pro-Gly | - |
? | |
3.4.19.3 | pyroglutamyl-His-Pro-Gly-NH2 + H2O | - |
Bos taurus | pyroglutamate + His-Pro-Gly-NH2 | - |
? | |
3.4.19.3 | pyroglutamyl-Phe-Pro-NH2 + H2O | - |
Bos taurus | pyroglutamate + Phe-Pro-NH2 | - |
? | |
3.4.19.3 | thyroliberin + H2O | - |
Bos taurus | pyroglutamate + His-Pro-NH2 | - |
? | |
3.4.19.6 | pyroglutamyl-7-amido-4-methylcoumarin + H2O | - |
Bos taurus | pyroglutamate + 7-amino-4-methylcoumarin | - |
? | |
3.4.19.6 | pyroglutamyl-His-Pro-7-amido-4-methylcoumarin + H2O | - |
Bos taurus | pyroglutamate + His-Pro-7-amido-4-methylcoumarin | - |
? | |
3.4.19.6 | thyroliberin + H2O | - |
Bos taurus | pyroglutamic acid + His-Pro-NH2 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.4.19.6 | More | a number of protein bands varying in MW from 55000 to 141000 Da are detected by SDS-PAGE | Bos taurus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.19.3 | 45 | - |
- |
Bos taurus |
3.4.19.6 | 45 | - |
- |
Bos taurus |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.19.6 | 25 | 56 | 25°C: 16% of maximal activity, 37°C: 70% of maximal activity, 45°C: maximal activity | Bos taurus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.19.3 | 45 | - |
stable for at least 1 h | Bos taurus |
3.4.19.3 | 55 | - |
partial inactivation | Bos taurus |
3.4.19.3 | 60 | - |
2 min, total inactivation | Bos taurus |
3.4.19.6 | 4 | - |
24 h, purified enzyme, 60% loss of activity | Bos taurus |
3.4.19.6 | 45 | - |
1 h, stable | Bos taurus |
3.4.19.6 | 55 | - |
2 min, partial inactivation | Bos taurus |
3.4.19.6 | 60 | - |
1 min, complete inactivation | Bos taurus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.4.19.3 | 6.8 | 7.6 | - |
Bos taurus |
3.4.19.6 | 6.8 | 7.6 | - |
Bos taurus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.4.19.3 | 6 | 8.5 | pH 6.5: about 45% of maximal activity, pH 8.5: about 25% of maximal activity | Bos taurus |
3.4.19.6 | 5.8 | 8.4 | pH 5.8: about 45% of maximal activity, pH 8.4: about 20% of maximal activity | Bos taurus |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.4.19.3 | 4 | 9.2 | rapid inactivation below pH 4.0 and above pH 9.2 | Bos taurus |
3.4.19.6 | 4 | 8 | 37°C, 15 min, stable from pH 4.0-8.0 | Bos taurus |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.19.6 | 0.008 | - |
Luteinizing hormone releasing hormone | - |
Bos taurus | |
3.4.19.6 | 0.0081 | - |
pyroglutamyl-His-Trp-Ser-Tyr-Gly | 37°C | Bos taurus | |
3.4.19.6 | 0.0108 | - |
pyroglutamyl-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly | 37°C | Bos taurus | |
3.4.19.6 | 0.0194 | - |
pyroglutamyl-His-Trp-Ser-Tyr-Gly-Leu | 37°C | Bos taurus | |
3.4.19.6 | 0.0296 | - |
pyroglutamyl-His-Pro-Gly-NH2 | 37°C | Bos taurus | |
3.4.19.6 | 0.0403 | - |
pyroglutamyl-His-Pro-NH2 | 37°C | Bos taurus | |
3.4.19.6 | 0.0588 | - |
pyroglutamyl-His-Pro-Gly | 37°C | Bos taurus | |
3.4.19.6 | 0.0603 | - |
pyroglutamyl-His-Trp-Ser-Tyr-Gly-Leu-Arg | 37°C | Bos taurus | |
3.4.19.6 | 0.0731 | - |
pyroglutamyl-Phe-Pro-NH2 | 37°C | Bos taurus | |
3.4.19.6 | 0.236 | - |
pyroglutamyl-His-Pro | 37°C | Bos taurus | |
3.4.19.6 | 0.582 | - |
pyroglutamyl-His-Gly-NH2 | 37°C | Bos taurus | |
3.4.19.6 | 1.67 | - |
Pyroglutamyl-His-Gly | 37°C | Bos taurus |