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Literature summary extracted from
- Purification and properties of proteinase B from yeast (1981), Biochim. Biophys. Acta, 661, 124-135.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.4.21.48 | freezing and thawing in absence of glycerol results in total loss of activity | Saccharomyces cerevisiae |
| 3.4.21.48 | HgCl2, 10 mM, stabilizes during purification | Saccharomyces cerevisiae |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.21.48 | antipain | - |
Saccharomyces cerevisiae |  |
| 3.4.21.48 | Aprotinin | i.e. trasylol, weak | Saccharomyces cerevisiae | |
| 3.4.21.48 | chymostatin | - |
Saccharomyces cerevisiae | |
| 3.4.21.48 | DFP | - |
Saccharomyces cerevisiae | |
| 3.4.21.48 | Hg2+ | HgCl2 | Saccharomyces cerevisiae | |
| 3.4.21.48 | p-hydroxymercuribenzoate | - |
Saccharomyces cerevisiae | |
| 3.4.21.48 | PMSF | - |
Saccharomyces cerevisiae | |
| 3.4.21.48 | Trypsin inhibitor | weak | Saccharomyces cerevisiae |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.4.21.48 | vacuole | - |
Saccharomyces cerevisiae | 5773 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.48 | 33000 | - |
x * 33000, SDS-PAGE | Saccharomyces cerevisiae |
| 3.4.21.48 | 33700 | - |
equilibroum sedimentation | Saccharomyces cerevisiae |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.21.48 | Saccharomyces cerevisiae | - |
Saccharomyces carlsbergensis | - |
| 3.4.21.48 | Saccharomyces cerevisiae | - |
Saccharomyces cerevisiae | - |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.4.21.48 | side-chain modification | contains 8-9% neutral sugars and 1.5% amino sugars | Saccharomyces cerevisiae |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.21.48 | large scale | Saccharomyces cerevisiae |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.4.21.48 | -20°C, 20% glycerol, 0.1 mM HgCl2, stable for 2 months | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.21.48 | Azocoll + H2O | - |
Saccharomyces cerevisiae | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.21.48 | ? | x * 33000, SDS-PAGE | Saccharomyces cerevisiae |
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Release 2023.1 (January 2023)
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