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Literature summary extracted from

  • Glazer, A.N.; Smith, E.L.
    Papain and other plant sulfhydryl proteolytic enzymes (1971), The Enzymes, 3rd Ed. (Boyer, P. D. , ed. ), 3, 501-546.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.22.2 Cys activates Carica papaya
3.4.22.2 EDTA required for maximal activity Carica papaya
3.4.22.2 Reducing agents e.g. 2,3-dimercaptopropanol, activates Carica papaya
3.4.22.2 Sodium borohydride activates Carica papaya
3.4.22.2 thiol compounds activate Carica papaya

General Stability

EC Number General Stability Organism
3.4.22.2 remains fully active after prolonged exposure to 9 M urea Carica papaya

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.2 1,3-Dibromoacetone
-
 Carica papaya
3.4.22.2 Bromoacetic acid
-
 Carica papaya
3.4.22.2 DFP
-
 Carica papaya
3.4.22.2 iodoacetamide
-
 Carica papaya
3.4.22.2 iodoacetic acid
-
 Carica papaya
3.4.22.2 p-CH3-C6H4(SO2)-NH-CH2-CO-CH2Cl
-
 Carica papaya

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.22.2 0.008
-
 N-benzyloxycarbonyl-Gly p-nitrophenyl ester
-
 Carica papaya
3.4.22.2 10
-
 Nalpha-benzoyl-L-citrulline methyl ester
-
 Carica papaya
3.4.22.2 18
-
 Nalpha-benzoyl-L-Arg ethyl ester at 37°C Carica papaya
3.4.22.2 20
-
 Nalpha-benzyloxycarbonyl-L-histidinamide
-
 Carica papaya
3.4.22.2 21
-
 N-benzoylglycinamide 38°C Carica papaya
3.4.22.2 23
-
 Nalpha-benzoyl-L-Arg ethyl ester at 25°C Carica papaya
3.4.22.2 32
-
 Nalpha-benzoyl-L-argininamide 25°C Carica papaya
3.4.22.2 40
-
 Nalpha-benzoyl-L-argininamide 38°C Carica papaya
3.4.22.2 320
-
 N-benzyloxycarbonyl-Gly-Gly
-
 Carica papaya

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.2 23406
-
 x * 23406, calculation from amino acid sequence Carica papaya
3.4.22.2 23700
-
 equilibrium sedimentation Carica papaya

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.2 Carica papaya
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.2 juice maximal concentration is reached early in the development of the fruit Carica papaya
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.2 N-acetyl-L-tyrosinamide + H2O
-
 Carica papaya N-acetyl-L-Tyr + NH3
-
 ?
3.4.22.2 N-benzoylglycinamide + H2O
-
 Carica papaya Nalpha-benzoyl-Gly + NH3
-
 ?
3.4.22.2 N-benzyloxycarbonyl-Gly 2-nitrophenyl ester + H2O
-
 Carica papaya N-benzyloxycarbonyl-Gly + 2-nitrophenol
-
 ?
3.4.22.2 N-benzyloxycarbonyl-Gly 3-nitrophenyl ester + H2O
-
 Carica papaya N-benzyloxycarbonyl-Gly + 3-nitrophenol
-
 ?
3.4.22.2 N-Benzyloxycarbonyl-Gly 4-nitrophenyl ester + H2O
-
 Carica papaya N-Benzyloxycarbonyl-Gly + 4-nitrophenol
-
 ?
3.4.22.2 N-benzyloxycarbonyl-Gly ethyl ester + H2O
-
 Carica papaya ?
-
 ?
3.4.22.2 N-benzyloxycarbonyl-Gly phenyl ester + H2O
-
 Carica papaya ?
-
 ?
3.4.22.2 N-benzyloxycarbonyl-Gly-Gly + H2O
-
 Carica papaya ?
-
 ?
3.4.22.2 N-benzyloxycarbonyl-L-Glu diamide + H2O
-
 Carica papaya ?
-
 ?
3.4.22.2 N-benzyloxycarbonyl-L-histidinamide + H2O
-
 Carica papaya Nalpha-benzoyl-L-His + NH3
-
 ?
3.4.22.2 N-benzyloxycarbonyl-L-leucinamide + H2O
-
 Carica papaya Nalpha-benzoyl-L-Leu + NH3
-
 ?
3.4.22.2 Nalpha-Benzoyl-L-Arg ethyl ester + H2O
-
 Carica papaya ?
-
 ?
3.4.22.2 Nalpha-benzoyl-L-argininamide + H2O
-
 Carica papaya Nalpha-benzoyl-L-Arg + NH3
-
 ?
3.4.22.2 Nalpha-benzoyl-L-citrulline methyl ester + H2O
-
 Carica papaya ?
-
 ?
3.4.22.2 Nalpha-benzoyl-L-lysinamide + H2O
-
 Carica papaya Nalpha-benzoyl-L-Lys + NH3
-
 ?
3.4.22.2 Nalpha-benzyloxycarbonyl-L-histidinamide + H2O
-
 Carica papaya ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.22.2 ? x * 23406, calculation from amino acid sequence Carica papaya

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.22.2 additional information
-
 additional information
-
 Carica papaya

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.22.2 4
-
 rapid and irreversible inactivation, at elevated temperatures Carica papaya