1.1.1.4 4-Methylpyrazole - 2464 1.1.1.4 5,5'-dithiobis(2-nitrobenzoic acid) - 221 1.1.1.4 acetone 38.3% residual activity at 10% (v/v) 333 1.1.1.4 acetonitrile 2.4% residual activity at 10% (v/v) 981 1.1.1.4 Ag+ complete inhibition at 1 mM 75 1.1.1.4 Al3+ 21.6% residual activity at 1 mM 264 1.1.1.4 Ba2+ 85.8% residual activity at 1 mM 111 1.1.1.4 Borate buffer - 98436 1.1.1.4 Ca2+ 89.3% residual activity at 1 mM 15 1.1.1.4 Ca2+ inhibits (3S/3R)-acetoin reduction 15 1.1.1.4 Co2+ 79.5% residual activity at 1 mM 23 1.1.1.4 Cu2+ - 19 1.1.1.4 Cu2+ strong inhibition at 1 mM 19 1.1.1.4 Cu2+ complete inhibition at 1 mM 19 1.1.1.4 dipicolinate - 4005 1.1.1.4 DMSO 8.4% residual activity at 40% (v/v) 896 1.1.1.4 EDTA - 21 1.1.1.4 EDTA strong inhibition at 1 mM 21 1.1.1.4 ethanol 3.7% residual activity at 10% (v/v) 69 1.1.1.4 Fe2+ complete inhibition at 1 mM 25 1.1.1.4 Fe2+ strongly inhibits (3S/3R)-acetoin reduction by over 85% 25 1.1.1.4 Fe3+ slight inhibition at 1 mM 70 1.1.1.4 Fe3+ strongly inhibits (3S/3R)-acetoin reduction by over 85% 70 1.1.1.4 Hg2+ - 33 1.1.1.4 methanol 14% residual activity at 10% (v/v) 83 1.1.1.4 Mn2+ 81.1% residual activity at 1 mM 11 1.1.1.4 additional information nonionic detergent Triton X-100 and Tween 80, as well as DMSO have no significant effect on enzyme activity 2 1.1.1.4 Na+ slight inhibition at 1 mM 59 1.1.1.4 NADH NADH availability limits the 2,3-butanediol dehydrogenase reaction 8 1.1.1.4 o-phenanthroline - 239 1.1.1.4 Zn2+ - 14 1.1.1.4 Zn2+ 3.4% residual activity at 1 mM 14 1.1.1.4 Zn2+ inhibits (3S/3R)-acetoin reduction 14