1.3.1.86 2-mercaptoethanol at high concentrations beta-mercaptoethanol is inhibitory 63 1.3.1.86 2-methylcrotonyl-CoA CoA-activated enzyme shows competitive inhibition with the substrate analog 2-methylcrotonyl-CoA 5899 1.3.1.86 4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole extremely potent inhibitor 15549 1.3.1.86 5,5'-dithiobis(2-nitrobenzoic acid) extremely potent inhibitor 221 1.3.1.86 acetyl-CoA competitive inhibitor 29 1.3.1.86 ammonium sulfate enzyme activity is inhibited by ammonium sulfate ; however, this inhibition is overcome by addition of 10 mM guanidine 1058 1.3.1.86 arachidoyl-CoA 86% residual activity at 0.1 mM 2827 1.3.1.86 butyryl-CoA competitive inhibitor 270 1.3.1.86 butyryl-CoA slight inhibition 270 1.3.1.86 Ca2+ complete inhibition at 1 mM 15 1.3.1.86 Co2+ complete inhibition at 1 mM 23 1.3.1.86 dithiothreitol at high concentrations dithiothreitol is inhibitory 45 1.3.1.86 iodoacetamide - 67 1.3.1.86 iodoacetamide 40% inhibition at 1 mM 67 1.3.1.86 iodoacetate - 93 1.3.1.86 isomyristoyl-CoA 78% residual activity at 0.1 mM 83382 1.3.1.86 isopalmitoyl-CoA 95% residual activity at 0.1 mM 83381 1.3.1.86 malonyl-CoA noncompetitive inhibitor 76 1.3.1.86 Mg2+ 30% inhibition at 1 mM 6 1.3.1.86 Mn2+ complete inhibition at 1 mM 11 1.3.1.86 additional information no significant inhibition of the activity of crotonyl-CoA reductase is observed upon addition of either flavin adenine dinucleotide (0.018 and 0.072 mM) or flavin mononucleotide (0.013 and 0.130 mM) 2 1.3.1.86 myristoyl-CoA 36% residual activity at 0.1 mM 457 1.3.1.86 N-ethylmaleimide extremely potent inhibitor 49 1.3.1.86 N-ethylmaleimide 80% inhibition at 1 mM 49 1.3.1.86 NADP+ - 10 1.3.1.86 NADPH concentrations of NADPH above 0.2 mM lead to inhibition of enzyme activity 5 1.3.1.86 p-chloromercuribenzoate a 30-min incubation of crotonyl-CoA reductase with p-chloromercuribenzoate at 0.008 mM leads to approximately 8.5% inhibition of enzyme activity 43 1.3.1.86 palmitoyl-CoA 24% residual activity at 0.1 mM 128 1.3.1.86 stearoyl-CoA 92% residual activity at 0.1 mM 526 1.3.1.86 Zn2+ 55% inhibition at 1 mM 14