3.6.1.23 (E)-3-(2,2-difluoroethoxy)-N-(7-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-2-methylhept-5-en-2-yl)benzenesulfonamide - 88125 3.6.1.23 (E)-3-(cyclopentyloxy)-N-(7-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-2-methylhept-5-en-2-yl)benzenesulfonamide - 88124 3.6.1.23 (E)-3-(cyclopropylmethoxy)-N-(7-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-2-methylhept-5-en-2-yl)benzenesulfonamide - 88123 3.6.1.23 (E)-N-(7-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-2-methylhept-5-en-2-yl)-3-methoxybenzenesulfonamide - 88122 3.6.1.23 (E)-N-(7-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-2-methylhept-5-en-2-yl)benzenesulfonamide - 88117 3.6.1.23 (R)-1-(3-(2-(hydroxydiphenylmethyl)-pyrrolidin-1-ylsulfonyl)propyl)pyrimidine-2,4(1H,3H)-dione - 169872 3.6.1.23 (R)-1-(4-(2-(hydroxydiphenylmethyl)-pyrrolidin-1-yl)-4-oxobutyl)pyrimidine-2,4(1H,3H)-dione - 87057 3.6.1.23 (R)-N-(1-(3-(2,2-difluoroethoxy)phenyl)ethyl)-4-((2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)methyl)benzenesulfonamide - 88128 3.6.1.23 (R)-N-(1-(3-(cyclopentyloxy)phenyl)ethyl)-4-((2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)methyl)benzenesulfonamide - 88127 3.6.1.23 (R)-N-(1-(3-(cyclopropylmethoxy)phenyl)ethyl)-4-((2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)methyl)-benzenesulfonamide - 88126 3.6.1.23 (R,E)-N-(1-(3-(2,2-difluoroethoxy)phenyl)ethyl)-5-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)pent-3-ene-1-sulfonamide - 88131 3.6.1.23 (R,E)-N-(1-(3-(cyclopentyloxy)phenyl)ethyl)-5-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)pent-3-ene-1-sulfonamide - 88130 3.6.1.23 (R,E)-N-(1-(3-(cyclopropylmethoxy)phenyl)ethyl)-5-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)pent-3-ene-1-sulfonamide - 88129 3.6.1.23 (S)-1-(4-(2-(hydroxydiphenylmethyl)-pyrrolidin-1-yl)-4-oxobutyl)pyrimidine-2,4(1H,3H)-dione - 169871 3.6.1.23 1,10-phenanthroline 50 mM, 30% inhibition 62 3.6.1.23 1,10-phenanthroline - 62 3.6.1.23 1-(2-amido-4-trityloxybutyl)uracil - 88097 3.6.1.23 1-(2-dimethylamido-4-trityloxybutyl)uracil - 88098 3.6.1.23 1-(2-morpholinamido-4-trityloxybutyl)uracil - 88102 3.6.1.23 1-(3-tert-butyldimethylsilyloxypropyl)uracil - 24136 3.6.1.23 1-(3-triphenylsilyloxypropyl)uracil - 24135 3.6.1.23 1-(3-tritylaminopropyl)uracil - 24134 3.6.1.23 1-(3-trityloxypropyl)uracil - 24133 3.6.1.23 1-(3-[[(2S)-2-[hydroxy[bis(2-methoxyphenyl)]methyl]pyrrolidin-1-yl]sulfonyl]propyl)pyrimidine-2,4(1H,3H)-dione - 87033 3.6.1.23 1-(3-[[(2S)-2-[hydroxy[bis(3-methoxyphenyl)]methyl]pyrrolidin-1-yl]sulfonyl]propyl)pyrimidine-2,4(1H,3H)-dione - 87034 3.6.1.23 1-(4-[(2S)-2-[bis(3-chlorophenyl)(hydroxy)methyl]pyrrolidin-1-yl]-4-oxobutyl)pyrimidine-2,4(1H,3H)-dione - 87048 3.6.1.23 1-(4-[(2S)-2-[bis(3-fluorophenyl)(hydroxy)methyl]pyrrolidin-1-yl]-4-oxobutyl)pyrimidine-2,4(1H,3H)-dione - 87032 3.6.1.23 1-(4-[(2S)-2-[bis(4-chlorophenyl)(hydroxy)methyl]pyrrolidin-1-yl]-4-oxobutyl)pyrimidine-2,4(1H,3H)-dione - 87023 3.6.1.23 1-(4-[(2S)-2-[bis(4-fluorophenyl)(hydroxy)methyl]pyrrolidin-1-yl]-4-oxobutyl)pyrimidine-2,4(1H,3H)-dione - 87050 3.6.1.23 1-(5-triphenylsilyloxypentyl)uracil - 24139 3.6.1.23 1-(5-tritylaminopentyl)uracil - 24138 3.6.1.23 1-(5-trityloxypentyl)uracil - 24137 3.6.1.23 1-[(1E,8E)-5-([[(E)-(3,4-dihydroxy-5-methoxybenzylidene)amino]oxy]methyl)-1-(3,4-dihydroxy-5-methoxyphenyl)-3,7-dioxa-2,8-diazadeca-1,8-dien-10-yl]pyrimidine-2,4(1H,3H)-dione - 169201 3.6.1.23 1-[(1Z,8E)-5-([[(E)-(3,4-dihydroxy-5-methoxybenzylidene)amino]oxy]methyl)-1-(3,4-dihydroxy-5-methoxyphenyl)-3,7-dioxa-2,8-diazadeca-1,8-dien-10-yl]pyrimidine-2,4(1H,3H)-dione - 87035 3.6.1.23 1-[(2E)-4-(trityloxy)but-2-en-1-yl]pyrimidine-2,4(1H,3H)-dione - 19626 3.6.1.23 1-[(2R,5S)-5-([[tert-butyl(diphenyl)silyl]oxy]methyl)-2,5-dihydrofuran-2-yl]pyrimidine-2,4(1H,3H)-dione - 19627 3.6.1.23 1-[(2R,5S)-5-[(trityloxy)methyl]-2,5-dihydrofuran-2-yl]pyrimidine-2,4(1H,3H)-dione - 19628 3.6.1.23 1-[(E)-4-trityloxy-2-butenyl]uracil - 24141 3.6.1.23 1-[(Z)-4-trityloxy-2-butenyl]uracil - 24140 3.6.1.23 1-[2-(acetamido)-4-(tert-butyldiphenylsilyloxy)butyl]uracil - 88108 3.6.1.23 1-[2-(acetamido)-4-(triphenylmethyl)aminobutyl]uracil - 88115 3.6.1.23 1-[2-(azidomethyl)-4-(trityloxy)butyl]uracil - 24147 3.6.1.23 1-[2-(carbobenzoxymethyl)-4-(triphenylmethyl)aminobutyl]uracil - 88113 3.6.1.23 1-[2-(carboethoxyethylamido)-4-trityloxybutyl]uracil - 88100 3.6.1.23 1-[2-(carboxy)-4-(tert-butyldiphenylsilyloxy)butyl]uracil - 88107 3.6.1.23 1-[2-(carboxy)-4-(triphenylmethyl)aminobutyl]uracil - 88114 3.6.1.23 1-[2-(carboxyethylamido)-4-trityloxybutyl]uracil - 88106 3.6.1.23 1-[2-(diethylamido)-4-(tert-butyldiphenylsilyloxy)butyl]uracil - 88111 3.6.1.23 1-[2-(ethylamido)-4-(tert-butyldiphenylsilyloxy) butyl]uracil - 88110 3.6.1.23 1-[2-(hydroxyethylamido)-4-trityloxybutyl]uracil - 88105 3.6.1.23 1-[2-(hydroxymethyl)-4-(trityloxy)butyl]uracil - 24145 3.6.1.23 1-[2-(methoxyethylamido)-4-trityloxybutyl]uracil - 88103 3.6.1.23 1-[2-(methylamido)-4-(tert-butyldiphenylsilyloxy)butyl]uracil - 88109 3.6.1.23 1-[2-(N,N-dimethylaminoethylamido)-4-trityloxybutyl]uracil - 88101 3.6.1.23 1-[2-(N-benzyloxycarbonylpiperazinamido)-4-(tert-butyldiphenylsilyloxy)butyl]uracil - 88112 3.6.1.23 1-[2-(N-methylpiperazine)amido-4-trityloxybutyl]uracil - 88104 3.6.1.23 1-[2-(tert-butyldiphenylsilyloxyethylamido)-4-trityloxybutyl]uracil - 88099 3.6.1.23 1-[2-(trityloxy)ethoxymethyl]uracil - 24132 3.6.1.23 1-[3-(4-benzylpiperidin-1-yl)-3-oxopropyl]pyrimidine-2,4(1H,3H)-dione - 41995 3.6.1.23 1-[3-(benzhydrylaminocarbonyl)propyl]uracil 1429fold selectivity over human enzyme 205964 3.6.1.23 1-[3-(tritylamino)propyl]pyrimidine-2,4(1H,3H)-dione - 19631 3.6.1.23 1-[3-(tritylaminocarbonyl)propyl]uracil 4545fold selectivity over human enzyme 205965 3.6.1.23 1-[3-([(2R)-2-[hydroxy(diphenyl)methyl]pyrrolidin-1-yl]sulfonyl)propyl]pyrimidine-2,4(1H,3H)-dione - 87041 3.6.1.23 1-[3-([(2S)-2-[bis(3-fluorophenyl)(hydroxy)methyl]pyrrolidin-1-yl]sulfonyl)propyl]pyrimidine-2,4(1H,3H)-dione - 87031 3.6.1.23 1-[3-([(2S)-2-[bis(4-fluorophenyl)(hydroxy)methyl]pyrrolidin-1-yl]sulfonyl)propyl]pyrimidine-2,4(1H,3H)-dione - 87022 3.6.1.23 1-[3-[(2S)-2-(diphenylmethyl)pyrrolidin-1-yl]-3-oxopropyl]pyrimidine-2,4(1H,3H)-dione - 87029 3.6.1.23 1-[4-(tert-butoxycarbonylamino)-3-(trityloxymethyl)butyl]uracil - 24144 3.6.1.23 1-[4-acetoxy-3-(tritylaminomethyl)butyl]uracil - 24142 3.6.1.23 1-[4-hydroxy-2-(trityloxymethyl)butyl]uracil - 24146 3.6.1.23 1-[4-hydroxy-3-(tritylaminomethyl)butyl]uracil - 24143 3.6.1.23 1-[4-hydroxy-3-[(tritylamino)methyl]butyl]pyrimidine-2,4(1H,3H)-dione - 19630 3.6.1.23 1-[4-[(2S)-2-(diphenylmethyl)pyrrolidin-1-yl]-4-oxobutyl]pyrimidine-2,4(1H,3H)-dione - 87028 3.6.1.23 1-[4-[(2S)-2-[bis[2-(benzyloxy)phenyl](hydroxy)methyl]pyrrolidin-1-yl]-4-oxobutyl]pyrimidine-2,4(1H,3H)-dione - 87024 3.6.1.23 1-[4-[(2S)-2-[bis[3-(benzyloxy)phenyl](hydroxy)methyl]pyrrolidin-1-yl]-4-oxobutyl]pyrimidine-2,4(1H,3H)-dione - 87025 3.6.1.23 1-[4-[(2S)-2-[bis[4-(benzyloxy)phenyl](hydroxy)methyl]pyrrolidin-1-yl]-4-oxobutyl]pyrimidine-2,4(1H,3H)-dione - 87026 3.6.1.23 1-[4-[(2S)-2-[hydroxy[di(thiophen-3-yl)]methyl]pyrrolidin-1-yl]-4-oxobutyl]pyrimidine-2,4(1H,3H)-dione - 87027 3.6.1.23 1-[6-(tritylamino)hexyl]pyrimidine-2,4(1H,3H)-dione - 19629 3.6.1.23 1-[N-(2-triphenylmethylaminoethyl)-acetamide]uracil - 39109 3.6.1.23 1-[N-(3-triphenylmethylaminopropyl)-acetamide]uracil - 39110 3.6.1.23 1-[N-(4-triphenylmethylaminobutyl)-acetamide]uracil - 39111 3.6.1.23 1-[N-(triphenylmethyl) diaminodi(n-propyl) acetamide]uracil - 39113 3.6.1.23 1-[N-(triphenylmethylaminoethyl)-N-(aminoethyl) acetamide]uracil - 39112 3.6.1.23 1-[N-hydroxyethyl-N-(triphenylmethylamino) ethyl-acetamide]uracil - 39114 3.6.1.23 1-[[2-(trityloxy)ethoxy]methyl]pyrimidine-2,4(1H,3H)-dione - 10359 3.6.1.23 2',3',5'-trideoxy-3'-fluoro-5'-(tritylamino)uridine activity against the parasites with IC50: 0.0053 mM 35733 3.6.1.23 2',3'-didehydro-2',3'-dideoxyuridine 5'-diphenyl phosphate - 11681 3.6.1.23 2',3'-dideoxy-3'-fluoro-5'-O-trityluridine activity against the parasites with IC50: 0.0020 mM, binding structure involving Tyr112, Ile117, Ile108, and Asn103, overview 9900 3.6.1.23 2',3'-dideoxy-3'-fluoro-5'-O-trityluridine - 9900 3.6.1.23 2',5'-dideoxy-5'-(tritylamino)uridine activity against the parasites with IC50: 0.0045 mM 9899 3.6.1.23 2',5'-dideoxy-5'-(tritylamino)uridine - 9899 3.6.1.23 2',5'-dideoxyuridine 5'-N-diphenylphosphoramidate - 11673 3.6.1.23 2'-deoxy-5'-O-(triphenylsilyl)uridine activity against the parasites with IC50: 0.0011 mM 35732 3.6.1.23 2'-deoxy-5'-O-trityluridine activity against the parasites with IC50: 0.006 mM 23886 3.6.1.23 2'-deoxy-5'-O-trityluridine - 23886 3.6.1.23 2'-deoxy-5'-O-[tris(1-methylethoxy)silyl]uridine activity against the parasites with IC50: 0.013 mM 35734 3.6.1.23 2'-deoxy-5'-phenylmethylaminouracil - 73880 3.6.1.23 2'-deoxy-5'-triphenylmethanyluracil - 73877 3.6.1.23 2'-deoxyuracil 5'-diphenyl phosphate - 73871 3.6.1.23 2'-deoxyuridine competitive inhibition 1705 3.6.1.23 2'-deoxyuridine 5'-(alpha,beta-imido)triphosphate - 13108 3.6.1.23 2'-deoxyuridine 5'-(alpha,beta-imido)triphosphate best inhibitor 13108 3.6.1.23 3'-azido-2',3'-dideoxy-UTP competitive inhibitor 83754 3.6.1.23 3'-deoxy-5'-O-(hydroxy{[hydroxy(phosphonooxy)phosphoryl]amino}phosphoryl)uridine - 69273 3.6.1.23 3'-deoxy-5'-O-(hydroxy{[hydroxy(phosphonooxy)phosphoryl]amino}phosphoryl)uridine competitive inhibition 69273 3.6.1.23 3'-deoxy-5'-O-[hydroxy(phosphonoamino)phosphoryl]uridine competitive inhibition 144063 3.6.1.23 3'-O-tert-butyldimethylsilyl-2',5'-dideoxyuridine 5'-N-diphenylphosphoramidate - 11677 3.6.1.23 3'-O-tert-butyldimethylsilyl-5'-O-sulfamoyl-2'-deoxyuridine - 11676 3.6.1.23 3-(2,2-difluoroethoxy)-N-(2-(4-((2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)methyl)phenyl)propan-2-yl)-benzenesulfonamide - 88121 3.6.1.23 3-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-N-(2,4,4-trimethylpentan-2-yl)propanamide - 86998 3.6.1.23 3-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-N-(2-methylbutan-2-yl)propanamide - 87000 3.6.1.23 3-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-N-(2-phenylethyl)propanamide - 87058 3.6.1.23 3-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-N-(3-ethylhexan-3-yl)propanamide - 86999 3.6.1.23 3-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-N-(3-phenylpropyl)propanamide - 87012 3.6.1.23 3-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-N-(diphenylmethyl)propanamide - 87030 3.6.1.23 3-(cyclopentyloxy)-N-(2-(4-((2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)methyl)phenyl)propan-2-yl)-benzenesulfonamide - 88120 3.6.1.23 3-(cyclopropylmethoxy)-N-(2-(4-((2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)methyl)phenyl)propan-2-yl)-benzenesulfonamide - 88119 3.6.1.23 3-(cyclopropylmethoxy)-N-[5-[(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)methoxy]-2-methylpentan-2-yl]benzenesulfonamide - 87038 3.6.1.23 3-[5-(trityloxy)tetrahydrofuran-2-yl]pyridine-2,6(1H,3H)-dione - 73872 3.6.1.23 4-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-N-(1-hydroxy-2-methyl-1,1-diphenylpropan-2-yl)butanamide - 87051 3.6.1.23 4-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-N-(1-hydroxy-2-methylpropan-2-yl)butanamide - 87055 3.6.1.23 4-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-N-(2,2-diphenylethyl)-N-methylbutanamide - 87052 3.6.1.23 4-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-N-(2,2-diphenylethyl)butanamide - 87042 3.6.1.23 4-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-N-(2,4,4-trimethylpentan-2-yl)butanamide - 87044 3.6.1.23 4-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-N-(2-hydroxy-2,2-diphenylethyl)-N-methylbutanamide - 87056 3.6.1.23 4-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-N-(2-methyl-1-phenylpropan-2-yl)butanamide - 87046 3.6.1.23 4-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-N-(2-methyl-4-phenylbutan-2-yl)butanamide - 87054 3.6.1.23 4-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-N-(2-phenylpropan-2-yl)butanamide - 87047 3.6.1.23 4-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-N-(3-ethylpentan-3-yl)butanamide - 87059 3.6.1.23 4-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-N-(diphenylmethyl)butanamide - 87049 3.6.1.23 4-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-N-phenylbutanamide - 87043 3.6.1.23 4-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-N-[2-methyl-1-(naphthalen-2-yl)propan-2-yl]butanamide - 87053 3.6.1.23 4-hydroxymercuribenzoate 5 mM, complete inhibition 970 3.6.1.23 5'-(2,2-dimethyl-1,1-diphenylpropan-1-yl)-2',3'-dideoxyuracil - 73874 3.6.1.23 5'-(2,2-dimethyl-1,1-diphenylpropan-1-yl)-2'-deoxyuracil - 73878 3.6.1.23 5'-(2,2-dimethyl-1,1-diphenylpropan-1-yl)-3'-methyl-2',3'-dideoxyuracil - 73876 3.6.1.23 5'-(2,3,3-trimethylbutan-2-yl)-3'-methyl-2',3'-dideoxyuracil - 73875 3.6.1.23 5'-(2,4-dimethyl-3-(1-methylethyl)pentan-3-yl)-2'-deoxyuracil - 73879 3.6.1.23 5'-(P3-benzyl-alpha,beta-methylene)-2'deoxyuridine - 169200 3.6.1.23 5'-adamantoyl-3'-methyl-2',3'-dideoxyuracil - 38720 3.6.1.23 5'-hexadecanoyl-3'-methyl-2',3'-dideoxyuracil - 73873 3.6.1.23 5'-O-(1-adamantoyl)-2',3'-didehydro-2',3'-dideoxyuridine - 11683 3.6.1.23 5'-O-(4,4'-dimethoxytrityl)-2'-deoxyuridine - 56236 3.6.1.23 5'-O-sulfamoylcarbamoyl-2',3'-didehydro-2',3'-dideoxyuridine - 11682 3.6.1.23 5'-O-triphenylsilyl-2',3'-didehydro-2',3'-dideoxyuridine - 11684 3.6.1.23 5'-O-triphenylsilyl-2',3'-dideoxy-3'-fluorouridine - 11679 3.6.1.23 5'-O-triphenylsilyl-2'-deoxyuridine - 11672 3.6.1.23 5'-pentanoylamino-2',5'-dideoxyuridine - 11674 3.6.1.23 5'-tert-butyldiphenylsilyloxy-2',3'-dideoxy-3'-fluorouridine - 11678 3.6.1.23 5'-tritylamino-2',5'-dideoxyuridine - 11675 3.6.1.23 5'-tritylamino-3'-fluoro-2',3',5'-trideoxyuridine - 11680 3.6.1.23 5-Aminouracil 5 mM, 50% loss of activity 12380 3.6.1.23 6-aminouracil 5 mM, 60% loss of activity 21523 3.6.1.23 8-azido-ATP competitive 4776 3.6.1.23 Ag+ - 75 3.6.1.23 alpha,beta-imido-dUDP - 34637 3.6.1.23 alpha,beta-imido-dUDP potent inhibition 34637 3.6.1.23 alpha,beta-imido-dUTP - 8742 3.6.1.23 alpha,beta-imido-dUTP substrate analogue, inhibits the wild-type enzyme, not mutant S72A 8742 3.6.1.23 alpha,beta-imido-dUTP competitive inhibition 8742 3.6.1.23 alpha-beta-imido-dUTP competitive, strong inhibition 56235 3.6.1.23 Ba2+ - 111 3.6.1.23 bacteriophage PBS2-induced inhibitor seems to form a high MW complex with the Bacillus subtilis dUTPase, the lower MW enzyme form of dUTPase is not inactivated by the protease inhibitor 105506 3.6.1.23 beta-hydroxyisovalerylshikonin an ATP-noncompetitive inhibitor of protein tyrosine kinases, induces apoptosis in human lung cancer DMS114 cells through reduction of dUTP nucleotidohydrolase activity, acts synergistically with 5-fluorouracil, overview 25465 3.6.1.23 bovine serum albumin 38% inhibition at 0.01% 274 3.6.1.23 Ca2+ 5 mM, 69% inhibition 15 3.6.1.23 Ca2+ - 15 3.6.1.23 Cd2+ - 52 3.6.1.23 Cu2+ 0.5 mM, 50% inhibition 19 3.6.1.23 Cu2+ - 19 3.6.1.23 dCTP - 179 3.6.1.23 deoxyuridine - 1060 3.6.1.23 diphosphate - 17 3.6.1.23 dTMP - 635 3.6.1.23 dTTP - 145 3.6.1.23 dTTP competitive feedback inhibition 145 3.6.1.23 dUDP - 1787 3.6.1.23 dUDP competitive 1787 3.6.1.23 dUDP binding structure 1787 3.6.1.23 dUDP competitive inhibition 1787 3.6.1.23 dUMP - 602 3.6.1.23 dUMP weak 602 3.6.1.23 dUMP competitive 602 3.6.1.23 dUMP product inhibition 602 3.6.1.23 dUMP product, competitive inhibition 602 3.6.1.23 dUMP alphaS - 105988 3.6.1.23 dUTP substrate inhibition at higher concentrations 435 3.6.1.23 dUTP - 435 3.6.1.23 dUTPalphaS - 32212 3.6.1.23 EDTA - 21 3.6.1.23 EDTA 2 mM, complete inhibition 21 3.6.1.23 EDTA reversed by Mg2+ or Zn2+ 21 3.6.1.23 EDTA 1 mM, 91% inhibition 21 3.6.1.23 EDTA 0.5 mM, more than 90% inhibition 21 3.6.1.23 EDTA dissociation of the enzyme into its monomeric form 21 3.6.1.23 EDTA reversed by Mg2+, Mn2+, Zn2+, Co2+ and Ca2+ 21 3.6.1.23 EDTA reversed by Mg2+, Co2+ or Mn2+ 21 3.6.1.23 EDTA reversed by Co2+ or Mg2+ 21 3.6.1.23 EDTA reversed by Mg2+, Mn2+ or Zn2+ 21 3.6.1.23 EDTA 1 mM, 65% loss of activity 21 3.6.1.23 EDTA causes a 105fold decrease and a 12fold increase of the kcat and Km values for dUTP hydrolysis, respectively, compared to the dUTP-Mg2+ complex 21 3.6.1.23 EDTA less than 10% residual activity at 1 mM 21 3.6.1.23 EGTA 10 mM, more than 90% inhibition 173 3.6.1.23 EGTA - 173 3.6.1.23 fluorodeoxy-UMP - 41264 3.6.1.23 guanidine hydrochloride kinetics of GuHCl-induced denaturation of the two dUTPase isozymes at pH 7.5, 4 °C and 1.5-4 M 1048 3.6.1.23 Hg+ - 1756 3.6.1.23 Hg2+ 10 mM HgCl2, 50% inhibition in absence of mercaptoethanol 33 3.6.1.23 Hg2+ - 33 3.6.1.23 KCl 0.4 mM, 29% loss of activity 79 3.6.1.23 Mg2+ complete inhibition by extensive dialysis against 0.0125 M MgCl2 6 3.6.1.23 Mn2+ 5 mM, 26% inhibition 11 3.6.1.23 Mn2+ - 11 3.6.1.23 additional information diphosphate has no effect on activity 2 3.6.1.23 additional information inhibitor in vivo activity against the parasite, overview 2 3.6.1.23 additional information growth inhibition potency in vitro, overview, molecular docking modeling study 2 3.6.1.23 additional information no inhibition by dTTp, dGTP, dATP, and dCTP 2 3.6.1.23 additional information the enzyme possesses a specific tryptic cleavage site Arg132-Ile133 20 A far from the active site of the enzyme, the enzyme is protected against tryptic digestion by binding alpha,beta-imino-dUTP which induces an allosteric conformational change within the central threefold channel of the homotrimer 2 3.6.1.23 additional information binding of alpha,beta-imino-dUTP does not induce allosteric conformational changes and does not protect the enzyme against tryptic digestion 2 3.6.1.23 additional information programmed cell death protein 4 downregulates dUTPase in certain cell types, overview 2 3.6.1.23 additional information fast screening for binding of potential inhibitors to the active site 2 3.6.1.23 additional information enzyme inhibitor development by high-throughput screening of triskelion libraries, a uracil-aldehyde ligand is covalently tethered to one position of a trivalent alkyloxyamine linker via an oxime linkage, and then the vacant linker positions are derivatized with a library of aldehydes, screening of triskelion oximes for inhibitory potency, overview 2 3.6.1.23 additional information computational docking of small molecules structures to the enzyme for inhibitor development, overview 2 3.6.1.23 additional information design and synthesis of tert-butyl diphenyl silyl uracil acetamides inhibitors, molecular modelling, overview 2 3.6.1.23 additional information several dUMP analogues with substituents at the 3'- or 5'-positions show poor inhibitory activity 2 3.6.1.23 additional information the enzyme is not inhibited by 3'-azido-2',3'-dideoxy-UTP 2 3.6.1.23 additional information the wild type enzyme is not inhibited by staphylococcal repressor StlSaPIbov1 protein 2 3.6.1.23 N,N-dibenzyl-3-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)propanamide - 87001 3.6.1.23 N-(2-(4-((2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)methyl)-phenyl)propan-2-yl)-3-methoxybenzenesulfonamide - 88118 3.6.1.23 N-(2-(4-((2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)methyl)-phenyl)propan-2-yl)benzenesulfonamide - 88116 3.6.1.23 N-benzyl-4-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)butanamide - 87045 3.6.1.23 N-tert-butyl-4-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)butanamide - 169870 3.6.1.23 N-[(1R)-1-[3-(cyclopropylmethoxy)-4-fluorophenyl]ethyl]-3-[(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)methoxy]propane-1-sulfonamide - 87036 3.6.1.23 N-[(1R)-1-[3-(cyclopropylmethoxy)phenyl]ethyl]-3-[(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)methoxy]propane-1-sulfonamide - 86992 3.6.1.23 N-[(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)acetyl]-D-alanyl-L-tyrosine - 69280 3.6.1.23 N-[(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)acetyl]-L-alanyl-L-aspartic acid - 69274 3.6.1.23 N-[(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)acetyl]-L-alanyl-L-glutamic acid - 69276 3.6.1.23 N-[(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)acetyl]-L-alanyl-L-glutamine - 69275 3.6.1.23 N-[(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)acetyl]-L-alanyl-L-lysine - 69278 3.6.1.23 N-[(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)acetyl]-L-alanyl-L-threonine - 69279 3.6.1.23 N-[(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)acetyl]-L-alanylglycine - 69277 3.6.1.23 N-[(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)acetyl]glycyl-L-lysine - 69282 3.6.1.23 N-[(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)acetyl]glycyl-L-threonine - 69283 3.6.1.23 N-[(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)acetyl]glycyl-L-tyrosine - 69284 3.6.1.23 N-[(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)acetyl]glycylglycine - 69281 3.6.1.23 N-[2-[3-(cyclopropylmethoxy)phenyl]propan-2-yl]-3-[(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)methoxy]propane-1-sulfonamide - 87039 3.6.1.23 N-[3-(tert-butyl diphenyl silanyloxy)-propyl]-2-uracil acetamide - 39107 3.6.1.23 N-[4-(tert-butyl diphenyl silanyloxy)-butyl]-2-uracil acetamide - 39108 3.6.1.23 N-[5-[(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)methoxy]-2-methylpentan-2-yl]benzenesulfonamide - 87037 3.6.1.23 NaF - 235 3.6.1.23 NEM 12 mM, 14% loss of activity 89 3.6.1.23 Pb2+ - 139 3.6.1.23 siRNAdUT3 a 21 base-pair double-stranded RNA molecule targeted against the motif 3 of the enzyme, time- and dose-dependent decrease in the dUTPase activity in transfected cells 132690 3.6.1.23 staphylococcal repressor protein StlSaPIBov1 highly potent inhibitor 247574 3.6.1.23 Stl protein - 247573 3.6.1.23 Stl repressor protein from Staphylococcus aureus - 247575 3.6.1.23 thymidine 5'-(alpha,beta-imido)triphosphate - 155730 3.6.1.23 thymidine triphosphate binding of thymidine triphosphate leads to disordered C-terminal arranged as a lid covering the active site, and the enzyme adapts an inactive conformation as a result of structural changes in the active site, mechanism, overview 144064 3.6.1.23 UDP - 26 3.6.1.23 Uracil 5 mM, 30% loss of activity 334 3.6.1.23 Uracil - 334 3.6.1.23 Zn2+ 5 mM, 23% inhibition 14 3.6.1.23 Zn2+ - 14