1.1.1.88 additional information - additional information kinetic analysis, overview. The enzyme exhibits positive cooperativity toward the substrates of the reductive reaction, but the oxidative reaction exhibits unusual double-saturation kinetics, distinctive among characterized HMG-CoA reductases. The unusual kinetics may arise from the presence of multiple active oligomeric states, each with different Vmax values 740104 1.1.1.88 0.00734 - 3-hydroxy-3-methylglutaryl-CoA at pH 7.4, temperature not specified in the publication 760594 1.1.1.88 0.0285 - NADH at pH 7.4, temperature not specified in the publication 760594 1.1.1.88 0.03 - CoASH H381A mutant, oxidative acylation of mevalonate 287502 1.1.1.88 0.032 - NADH reductive deacylation to mevalonate 287496, 287497 1.1.1.88 0.039 - CoA oxidative acylation of mevalonate 287496, 287497 1.1.1.88 0.046 - CoA mevaldate oxidation 287496, 287497 1.1.1.88 0.05 - CoASH oxidative acylation of mevalonate 287499 1.1.1.88 0.05 - CoASH wild-type enzyme, oxidative acylation of mevalonate 287502 1.1.1.88 0.05 - DL-3-hydroxy-3-methylglutaryl-CoA reductive deacylation to mevalonate 287496, 287497 1.1.1.88 0.06 - NAD+ mevaldate oxidation 287496, 287497 1.1.1.88 0.072 - NADH pH and temperature not specified in the publication 740104 1.1.1.88 0.078 - (S)-3-hydroxy-3-methylglutaryl-CoA pH and temperature not specified in the publication 740104 1.1.1.88 0.1 - CoA oxidative acylation of mevalonate 287495 1.1.1.88 0.11 - CoASH H381Q mutant, oxidative acylation of mevalonate 287502 1.1.1.88 0.14 - NAD+ H381A mutant, oxidative acylation of mevalonate 287502 1.1.1.88 0.15 - R-mevalonate oxidative acylation of mevalonate 287498 1.1.1.88 0.17 - NAD+ mevaldate oxidation 287499 1.1.1.88 0.17 - NADH reductive deacylation to mevalonate 287499 1.1.1.88 0.2 - CoASH H381N mutant, oxidative acylation of mevalonate 287502 1.1.1.88 0.21 - NAD+ wild-type enzyme, oxidative acylation of mevalonate 287505 1.1.1.88 0.22 - mevalonate oxidative acylation of mevalonate 287499 1.1.1.88 0.22 - NAD+ wild-type enzyme, oxidative acylation of mevalonate 287502 1.1.1.88 0.27 - (R,S)-mevalonate H381A mutant, oxidative acylation of mevalonate 287502 1.1.1.88 0.27 - NAD+ oxidative acylation of mevalonate 287496, 287497 1.1.1.88 0.3 - DL-3-hydroxy-3-methylglutaryl-CoA reductive deacylation to mevalonate 287499 1.1.1.88 0.3 - mevalonate oxidative acylation of mevalonate 287496, 287497 1.1.1.88 0.35 - DL-mevalonate oxidative acylation of mevalonate 287495 1.1.1.88 0.35 - NAD+ oxidative acylation of mevalonate 287495 1.1.1.88 0.36 - NADH mevaldate reduction to mevalonate 287496, 287497 1.1.1.88 0.37 - NAD+ oxidative acylation of mevalonate 287499 1.1.1.88 0.45 - (R,S)-mevalonate wild-type enzyme, oxidative acylation of mevalonate 287502 1.1.1.88 0.49 - DL-mevalonate oxidative acylation of mevalonate 287495 1.1.1.88 0.5 - CoASH H381K mutant, oxidative acylation of mevalonate 287502 1.1.1.88 0.6 - NAD+ oxidative acylation of mevalonate 287495 1.1.1.88 0.61 - DL-mevaldate mevaldate oxidation 287496, 287497 1.1.1.88 0.61 - DL-mevaldate oxidative acylation of DL-mevaldate 287496 1.1.1.88 0.62 - NAD+ H381Q mutant, oxidative acylation of mevalonate 287502 1.1.1.88 0.71 - (R,S)-mevalonate H381Q mutant, oxidative acylation of mevalonate 287502 1.1.1.88 0.75 - (R,S)-mevalonate H381K mutant, oxidative acylation of mevalonate 287502 1.1.1.88 0.81 - NAD+ H381N mutant, oxidative acylation of mevalonate 287502 1.1.1.88 1.23 - NAD+ H381K mutant, oxidative acylation of mevalonate 287502 1.1.1.88 1.36 - DL-mevaldate mevaldate oxidation 287499 1.1.1.88 3.7 - (R,S)-mevalonate H381N mutant, oxidative acylation of mevalonate 287502 1.1.1.88 9 - DL-mevaldate DL-mevaldate reduction 287496 1.1.1.88 9 - DL-mevaldate mevaldate reduction to mevalonate 287496, 287497 1.1.1.88 36 - DL-mevaldate mevaldate reduction to mevalonate 287499 1.1.1.88 52 - NADP+ wild-type enzyme, oxidative acylation of mevalonate 287505