1.1.1.286 additional information - additional information kinetic mechanism, detailed overview 685232 1.1.1.286 additional information - additional information Michaelis-Menten kinetics 740021 1.1.1.286 0.0073 - homoisocitrate pH 8.0, 60°C, recombinant His6-tagged wild-type enzyme 740021 1.1.1.286 0.011 - homoisocitrate pH 8.0, 60°C, recombinant enzyme 738195 1.1.1.286 0.014 - isocitrate pH 8.0, 60°C, recombinant His6-tagged wild-type enzyme 740021 1.1.1.286 0.0164 - isocitrate 70°C, pH 7.8 660907 1.1.1.286 0.0183 - homoisocitrate 70°C, pH 7.8 660907 1.1.1.286 0.027 - isocitrate pH 8.0, 60°C, recombinant enzyme 738195 1.1.1.286 0.036 - NAD+ pH 8.0, 60°C, recombinant His6-tagged wild-type enzyme, with homoisocitrate 740021 1.1.1.286 0.037 - homoisocitrate pH 8.0, 60°C, recombinant His6-tagged mutant S80A 740021 1.1.1.286 0.042 - homoisocitrate pH 8.0, 60°C, recombinant His6-tagged mutant L81P 740021 1.1.1.286 0.05 - 3-isopropylmalate pH 8.0, 60°C, recombinant His6-tagged mutant S80A 740021 1.1.1.286 0.0771 - NAD+ 70°C, pH 7.8 660907 1.1.1.286 0.1 - NAD+ pH 8.0, 60°C, recombinant His6-tagged wild-type enzyme, with isocitrate 740021 1.1.1.286 0.16 - NAD+ pH 8.0, 60°C, recombinant His6-tagged mutant L83R, with homoisocitrate 740021 1.1.1.286 0.17 - homoisocitrate pH 8.0, 60°C, recombinant His6-tagged mutant L83R 740021 1.1.1.286 0.193 - NAD+ 30°C, pH 7.8 660918 1.1.1.286 0.21 - homoisocitrate pH 8.0, 60°C, recombinant enzyme 740000 1.1.1.286 0.211 - homoisocitrate 30°C, pH 7.8, wild-type 660918 1.1.1.286 0.25 - NAD+ pH 8.0, 60°C, recombinant His6-tagged mutant T71V, with isocitrate 740021 1.1.1.286 0.29 - isocitrate pH 8.0, 60°C, recombinant enzyme 740000 1.1.1.286 0.291 - isocitrate 30°C, pH 7.8, wild-type 660918 1.1.1.286 0.3 - isocitrate pH 8.0, 60°C, recombinant His6-tagged mutant S80A 740021 1.1.1.286 0.31 - 3-isopropylmalate pH 8.0, 60°C, recombinant His6-tagged wild-type enzyme 740021 1.1.1.286 0.32 - NAD+ pH 8.0, 60°C, recombinant His6-tagged mutant L81P, with homoisocitrate 740021 1.1.1.286 0.405 - isocitrate 60°C, pH 8.0 656127 1.1.1.286 0.46 - homoisocitrate pH 8.0, 60°C, recombinant His6-tagged mutant I82N 740021 1.1.1.286 0.465 - isocitrate 30°C, pH 7.8, A80 deletion mutant 660918 1.1.1.286 0.5 - isocitrate pH 8.0, 60°C, recombinant His6-tagged mutant T71V 740021 1.1.1.286 0.58 - isocitrate pH 8.0, 60°C, recombinant His6-tagged mutant L83R 740021 1.1.1.286 0.6 - NAD+ pH 8.0, 60°C, recombinant His6-tagged mutant L81P, with isocitrate 740021 1.1.1.286 0.62 - NAD+ pH 8.0, 60°C, recombinant His6-tagged mutant L83R, with isocitrate 740021 1.1.1.286 0.64 - NAD+ pH 8.0, 60°C, recombinant His6-tagged mutant I82N, with homoisocitrate 740021 1.1.1.286 0.68 - 3-isopropylmalate pH 8.0, 60°C, recombinant His6-tagged mutant L81P 740021 1.1.1.286 0.71 - 3-isopropylmalate pH 8.0, 60°C, recombinant His6-tagged mutant T71V 740021 1.1.1.286 0.76 - NAD+ pH 8.0, 60°C, recombinant His6-tagged mutant S80A, with isocitrate 740021 1.1.1.286 0.819 - homoisocitrate 30°C, pH 7.8, A80 deletion mutant 660918 1.1.1.286 0.92 - NAD+ pH 8.0, 60°C, recombinant His6-tagged mutant S80A, with homoisocitrate 740021 1.1.1.286 0.94 - homoisocitrate pH 8.0, 60°C, recombinant His6-tagged mutant T71V 740021 1.1.1.286 1 - homoisocitrate 30°C, pH 7.8, mutant R87V 660918 1.1.1.286 1 - NAD+ pH 8.0, 60°C, recombinant His6-tagged mutant T71V, with 3-isopropylmalate 740021 1.1.1.286 1.2 - isocitrate pH 8.0, 60°C, recombinant His6-tagged mutant L81P 740021 1.1.1.286 1.2 - NAD+ pH 8.0, 60°C, recombinant His6-tagged mutant S80A, with 3-isopropylmalate 740021 1.1.1.286 1.3 - NAD+ pH 8.0, 60°C, recombinant His6-tagged mutant L81P, with 3-isopropylmalate 740021 1.1.1.286 1.33 - 3-isopropylmalate 30°C, pH 7.8, wild-type 660918 1.1.1.286 1.5 - homoisocitrate 30°C, pH 7.8, mutant R87T 660918 1.1.1.286 1.5 - NAD+ pH 8.0, 60°C, recombinant His6-tagged mutant T71V, with homoisocitrate 740021 1.1.1.286 1.5 - NAD+ pH 8.0, 60°C, recombinant His6-tagged wild-type enzyme, with 3-isopropylmalate 740021 1.1.1.286 1.8 - NAD+ pH 8.0, 60°C, recombinant His6-tagged mutant I82N, with isocitrate 740021 1.1.1.286 2.2 - isocitrate pH 8.0, 60°C, recombinant His6-tagged mutant I82N 740021 1.1.1.286 7.486 - homoisocitrate 60°C, pH 8.0 656127