1.1.1.383 additional information - NADH Km-value for NADH is below 0.001 mM, pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate 732457 1.1.1.383 additional information - NADPH Km-value for NADPH is below 0.001 mM, pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate 732457 1.1.1.383 0.001 - NADPH wild-type, pH not specified in the publication, temperature not specified in the publication 739528 1.1.1.383 0.013 - NADPH mutant Q110V, pH 7.0, temperature not specified in the publication 738976 1.1.1.383 0.013 - NADPH wild-type, pH not specified in the publication, temperature not specified in the publication 739528 1.1.1.383 0.015 - NADH mutant V48L/R49P/K52L/S53D/E59K/T182S/E320K, pH not specified in the publication, temperature not specified in the publication 739528 1.1.1.383 0.024 - NADH pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate 732457 1.1.1.383 0.024 - NADPH mutant Q110A, pH 7.0, temperature not specified in the publication 738976 1.1.1.383 0.026 - NADH mutant A71S/R76D/S78D/Q110V/D146G/G185R/K433E , pH not specified in the publication, temperature not specified in the publication 739528 1.1.1.383 0.03 - NADH mutant A71S/R76D/S78D/Q110V, pH 7.0, temperature not specified in the publication 738976 1.1.1.383 0.03 - NADH mutant A71S/R76D/S78D/Q110V, pH not specified in the publication, temperature not specified in the publication 739528 1.1.1.383 0.031 - NADPH pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate 732457 1.1.1.383 0.039 - NADH pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate 732457 1.1.1.383 0.04 - NADH mutant A71S/R76D/S78D/Q110A, pH 7.0, temperature not specified in the publication 738976 1.1.1.383 0.04 - NADPH wild-type, pH 7.0, temperature not specified in the publication 738976 1.1.1.383 0.041 - NADPH wild-type, pH not specified in the publication, temperature not specified in the publication 739528 1.1.1.383 0.045 - NADH wild-type, pH not specified in the publication, temperature not specified in the publication 739528 1.1.1.383 0.046 - NADPH pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate 732457 1.1.1.383 0.047 - NADH mutant S61D/S63D/I95V, pH not specified in the publication, temperature not specified in the publication 739528 1.1.1.383 0.065 - NADH mutant S78D/Q110V, pH 7.0, temperature not specified in the publication 738976 1.1.1.383 0.065 - NADPH mutant S78D/Q110V, pH 7.0, temperature not specified in the publication 738976 1.1.1.383 0.075 - NADH mutant A71S/R76D/S78AD/Q110V, pH not specified in the publication, temperature not specified in the publication 739528 1.1.1.383 0.09 - NADH mutant R76D/Ser78D, pH not specified in the publication, temperature not specified in the publication 739528 1.1.1.383 0.108 - NADH mutant V48L/R49P/K52L/S53D, pH not specified in the publication, temperature not specified in the publication 739528 1.1.1.383 0.113 - NADH mutant S61D/S63D, pH not specified in the publication, temperature not specified in the publication 739528 1.1.1.383 0.128 - NADH mutant V48L/R49P/K52E/S53D, pH not specified in the publication, temperature not specified in the publication 739528 1.1.1.383 0.13 - NADH mutant S78D, pH 7.0, temperature not specified in the publication 738976 1.1.1.383 0.14 - NADH mutant Q110V, pH 7.0, temperature not specified in the publication 738976 1.1.1.383 0.28 - NADH mutant Q110A, pH 7.0, temperature not specified in the publication 738976 1.1.1.383 0.285 - NADH wild-type, pH not specified in the publication, temperature not specified in the publication 739528 1.1.1.383 0.415 - NADH wild-type, pH not specified in the publication, temperature not specified in the publication 739528 1.1.1.383 0.57 - NADPH mutant A71S/R76D/S78D/Q110A, pH 7.0, temperature not specified in the publication 738976 1.1.1.383 0.6 - NADPH mutant A71S/R76D/S78AD/Q110V, pH not specified in the publication, temperature not specified in the publication 739528 1.1.1.383 0.65 - NADPH mutant A71S/R76D/S78D/Q110V, pH 7.0, temperature not specified in the publication 738976 1.1.1.383 0.65 - NADPH mutant A71S/R76D/S78D/Q110V, pH not specified in the publication, temperature not specified in the publication 739528 1.1.1.383 0.66 - NADPH mutant S78D, pH 7.0, temperature not specified in the publication 738976 1.1.1.383 0.75 - NADPH mutant V48L/R49P/K52L/S53D/E59K/T182S/E320K, pH not specified in the publication, temperature not specified in the publication 739528 1.1.1.383 0.88 - NADPH mutant S61D/S63D, pH not specified in the publication, temperature not specified in the publication 739528 1.1.1.383 1 - NADPH mutant R76D/Ser78D, pH not specified in the publication, temperature not specified in the publication 739528 1.1.1.383 1 - NADPH mutant S61D/S63D/I95V, pH not specified in the publication, temperature not specified in the publication 739528 1.1.1.383 1 - NADPH mutant V48L/R49P/K52L/S53D, pH not specified in the publication, temperature not specified in the publication 739528 1.1.1.383 1.075 - NADH wild-type, pH not specified in the publication, temperature not specified in the publication 739528 1.1.1.383 1.08 - NADH wild-type, pH 7.0, temperature not specified in the publication 738976 1.1.1.383 1.18 - NADPH mutant V48L/R49P/K52E/S53D, pH not specified in the publication, temperature not specified in the publication 739528 1.1.1.383 1.4 - NADPH mutant A71S/R76D/S78D/Q110V/D146G/G185R/K433E , pH not specified in the publication, temperature not specified in the publication 739528