2.4.1.10 additional information - additional information - 671507 2.4.1.10 additional information - additional information 2.3 mM (sucrose) total, mutant enzyme S173A 678072 2.4.1.10 additional information - additional information 29.2 mM (sucrose) total, mutant enzyme R370A, a 4fold KM value decrease compared with the wild-type enzyme supported the interactions of Arg 370 with the 2-OH and 3-OH of the glucosyl residue in the active site 678072 2.4.1.10 additional information - additional information 31.9 mM (sucrose) total, mutant enzyme W94A, 9% of the catalytic efficiency of the wild-type 678072 2.4.1.10 additional information - additional information 32 mM (sucrose), 25°C, pH 5.0, total activity 687775 2.4.1.10 additional information - additional information 35.1 mM (sucrose) total, mutant enzyme N252H 678072 2.4.1.10 additional information - additional information 4.1 mM (sucrose) total, mutant enzyme N252A 678072 2.4.1.10 additional information - additional information 480.4 mM (sucrose) total, mutant enzyme W172A, 72fold increase of the KM of the wild-type 678072 2.4.1.10 additional information - additional information 51.9 mM (sucrose) total, mutant enzyme Y421A, 3%of the wild-type activity 678072 2.4.1.10 additional information - additional information 6.6 mM (sucrose) total, wild-type, pH 6.6, 37°C, 50 mM Sorensen’s phosphate buffer, 7.36 mg/l enzyme concentration, reaction time 60 min 678072 2.4.1.10 additional information - additional information 66.6 mM (sucrose), mutant enzyme L118A 678072 2.4.1.10 additional information - additional information 7.5 mM (sucrose) total, mutant enzyme N252G 678072 2.4.1.10 additional information - additional information 8.4 mM (sucrose) total, mutant enzyme N252D 678072 2.4.1.10 additional information - additional information at pHs higher than 7.0, Zymomonas mobilis levansucrase does not exhibit levan synthesis activity 687775 2.4.1.10 additional information - additional information kinetics of free and immobilized enzymes, overview 701567 2.4.1.10 additional information - additional information kinetics, overview 704960 2.4.1.10 additional information - additional information Michaelis-Menten kinetics 735800, 735888, 737179 2.4.1.10 additional information - additional information mutant enzyme D257A, no activity 678072 2.4.1.10 additional information - additional information mutant enzyme D95A, no activity 678072 2.4.1.10 additional information - additional information mutant enzyme E350A, nearly inactive 678072 2.4.1.10 additional information - additional information mutant enzyme E352A, no measureable activity 678072 2.4.1.10 additional information - additional information mutant enzyme R256A, nearly inactive 678072 2.4.1.10 additional information - additional information mutant enzyme S164K is inactive 689854 2.4.1.10 additional information - additional information mutants A344P and F414W have the same behavior like the wild-type 689854 2.4.1.10 additional information - additional information mutants, overview 488343 2.4.1.10 2 - sucrose hydrolytic activity, at pH 6.0 and 37°C 756360 2.4.1.10 2 3 sucrose total activity, at pH 6.0 and 37°C 756360 2.4.1.10 2.3 - sucrose pH 6.6, mutant enzyme S173A 678072 2.4.1.10 2.5 - sucrose mutant enzyme S164A, 0.5 U/ml of enzyme activity at 37°C and pH 6.0 689854 2.4.1.10 3 - sucrose total activity, at pH 6.0 and 37°C 756360 2.4.1.10 4 - sucrose hydrolytic activity, at pH 6.0 and 37°C 756360 2.4.1.10 4.1 - sucrose pH 6.6, mutant enzyme N252A 678072 2.4.1.10 6.6 - sucrose pH 6.6, wild-type enzyme 678072 2.4.1.10 7.4 - sucrose mutant enzyme I341V, 0.5 U/ml of enzyme activity at 37°C and pH 6.0 689854 2.4.1.10 8 - sucrose - 488314 2.4.1.10 8 - sucrose total activity, at pH 6.0 and 37°C 756360 2.4.1.10 8 - sucrose transfructosylating activity, at pH 6.0 and 37°C 756360 2.4.1.10 8 - sucrose wild-type, 0.5 U/ml of enzyme activity at 37°C and pH 6.0 689854 2.4.1.10 9 - sucrose hydrolytic activity, at pH 6.0 and 37°C 756360 2.4.1.10 9 - sucrose total activity, at pH 6.0 and 37°C 756360 2.4.1.10 10.5 - sucrose mutant D309N 488340 2.4.1.10 10.5 - sucrose mutant enzyme H243L, 0.5 U/ml of enzyme activity at 37°C and pH 6.0 689854 2.4.1.10 11.4 - sucrose wild-type enzyme 488340 2.4.1.10 11.9 - sucrose 30°C, pH 5.0, wild-type enzyme 671836 2.4.1.10 12 - sucrose - 488322 2.4.1.10 12.3 - sucrose 30°C, pH 5.0, mutant enzyme D135N 671836 2.4.1.10 13.1 - sucrose 30°C, pH 5.0, mutant enzyme C395S 671836 2.4.1.10 13.5 - sucrose at 37°C 488332 2.4.1.10 13.8 - sucrose 30°C, pH 5.0, mutant enzyme C339S 671836 2.4.1.10 14 - sucrose - 488321 2.4.1.10 17.1 - sucrose sucrose hydrolysis, pH 6.0-6.6, 60°C 736891 2.4.1.10 18.5 - sucrose sucrose hydrolysis, pH 6.0-6.6, 60°C 736891 2.4.1.10 19 - sucrose - 488321 2.4.1.10 19.6 - sucrose wild type enzyme, hydrolytic activity, at pH 6.0 and 50°C 756958 2.4.1.10 20 - sucrose hydrolytic activity, at pH 6.0 and 37°C 756360 2.4.1.10 21 - sucrose hydrolytic activity, at pH 6.0 and 37°C 756360 2.4.1.10 22.5 - sucrose - 684765 2.4.1.10 24.1 - sucrose sucrose hydrolysis, pH 6.0-6.6, 60°C 736891 2.4.1.10 26.6 - sucrose - 672335 2.4.1.10 28 - sucrose - 488309 2.4.1.10 29.3 - sucrose mutant enzyme R433A, 0.5 U/ml of enzyme activity at 37°C and pH 6.0 689854 2.4.1.10 30 - sucrose mutant enzyme R369K, 0.5 U/ml of enzyme activity at 37°C and pH 6.0 689854 2.4.1.10 30 - sucrose total activity, at pH 6.0 and 37°C 756360 2.4.1.10 31.9 - sucrose pH 6.6, mutant enzyme W94A 678072 2.4.1.10 33.6 - sucrose at pH 6.5 and 37°C 756954 2.4.1.10 36 - sucrose 25°C, pH 5.0, transfructosylation activity 687775 2.4.1.10 40 - sucrose 25°C, pH 5.0, levan synthesis activity is monitored by following the increase in turbidity at 600 nm that occurrs due to the insoluble levan formation 687775 2.4.1.10 40 - sucrose sucrose hydrolysis, wild-type 488325 2.4.1.10 44.46 - sucrose wild type enzyme, total activity, at pH 6.0 and 50°C 756958 2.4.1.10 44.74 - sucrose mutant enzyme K372Y, hydrolytic activity, at pH 6.0 and 50°C 756958 2.4.1.10 47.81 - D-glucose pH 6.0, 30°C, recombinant enzyme 704960 2.4.1.10 50 - sucrose - 488313 2.4.1.10 50.5 - sucrose at pH 6.5 and 37°C 756954 2.4.1.10 51.9 - sucrose pH 6.6, mutant enzyme Y421A 678072 2.4.1.10 64 - sucrose at pH 5.9 and 50°C 757042 2.4.1.10 66.6 - sucrose pH 6.6, mutant enzyme L118A 678072 2.4.1.10 77.91 - sucrose mutant enzyme K372Y, total activity, at pH 6.0 and 50°C 756958 2.4.1.10 86.69 - sucrose mutant enzyme K372A, hydrolytic activity, at pH 6.0 and 50°C 756958 2.4.1.10 110 - sucrose sucrose hydrolysis, mutant R331K 488325 2.4.1.10 113.13 - sucrose mutant enzyme K372A, transfructosylation activity, at pH 6.0 and 50°C 756958 2.4.1.10 117.2 - sucrose pH 6.5, 40°C 735800 2.4.1.10 118 - sucrose mutant enzyme E404K, at pH 6.5 and 45°C 757280 2.4.1.10 120 - sucrose sucrose hydrolysis, mutant R331H 488325 2.4.1.10 122 - sucrose - 488312 2.4.1.10 125 - sucrose wild-type enzyme 488343 2.4.1.10 127.96 - sucrose mutant enzyme N251W, transfructosylation activity, at pH 6.0 and 50°C 756958 2.4.1.10 127.96 - sucrose wild type enzyme, transfructosylation activity, at pH 6.0 and 50°C 756958 2.4.1.10 131 - sucrose at pH 6.0 and 37°C 756953 2.4.1.10 137.62 - sucrose mutant enzyme N251W/K372Y, transfructosylation activity, at pH 6.0 and 50°C 756958 2.4.1.10 154 - sucrose mutant enzyme R360S, 0.5 U/ml of enzyme activity at 37°C and pH 6.0 689854 2.4.1.10 160 - sucrose - 488330 2.4.1.10 160 - sucrose sucrose hydrolysis, pH 5.8-6.6, 60°C 736891 2.4.1.10 176.72 - sucrose mutant enzyme K372Y, transfructosylation activity, at pH 6.0 and 50°C 756958 2.4.1.10 180 - sucrose sucrose hydrolysis, mutants R331L and R331S 488325 2.4.1.10 193.4 - sucrose mutant enzyme K372A, total activity, at pH 6.0 and 50°C 756958 2.4.1.10 249 - sucrose mutant enzyme E404P, at pH 6.5 and 45°C 757280 2.4.1.10 273 - sucrose mutant enzyme E404M, at pH 6.5 and 45°C 757280 2.4.1.10 283 - sucrose mutant enzyme E404Q, at pH 6.5 and 45°C 757280 2.4.1.10 284 - sucrose mutant enzyme E404F, at pH 6.5 and 45°C 757280 2.4.1.10 286 - sucrose mutant enzyme E404H, at pH 6.5 and 45°C 757280 2.4.1.10 287 - sucrose mutant enzyme E404Y, at pH 6.5 and 45°C 757280 2.4.1.10 293 - sucrose mutant enzyme E404G, at pH 6.5 and 45°C 757280 2.4.1.10 294 - sucrose mutant enzyme E404W, at pH 6.5 and 45°C 757280 2.4.1.10 296 - sucrose mutant enzyme E404L, at pH 6.5 and 45°C 757280 2.4.1.10 296 - sucrose mutant enzyme E404N, at pH 6.5 and 45°C 757280 2.4.1.10 296 - sucrose mutant enzyme E404S, at pH 6.5 and 45°C 757280 2.4.1.10 297.3 - sucrose mutant enzyme G361F, 0.5 U/ml of enzyme activity at 37°C and pH 6.0 689854 2.4.1.10 305 - sucrose wild type enzyme, at pH 6.5 and 45°C 757280 2.4.1.10 309 - sucrose mutant enzyme E404V, at pH 6.5 and 45°C 757280 2.4.1.10 318 - sucrose mutant enzyme E404I, at pH 6.5 and 45°C 757280 2.4.1.10 319.4 - sucrose mutant enzyme Y429N, 0.5 U/ml of enzyme activity at 37°C and pH 6.0 689854 2.4.1.10 338 - sucrose mutant enzyme E404T, at pH 6.5 and 45°C 757280 2.4.1.10 346 - sucrose mutant enzyme E404D, at pH 6.5 and 45°C 757280 2.4.1.10 355 - sucrose mutant enzyme E404A, at pH 6.5 and 45°C 757280 2.4.1.10 370 - sucrose mutant enzyme E404C, at pH 6.5 and 45°C 757280 2.4.1.10 377 - sucrose mutant enzyme E404R, at pH 6.5 and 45°C 757280 2.4.1.10 436 - sucrose transfructosylating activity, at pH 6.0 and 37°C 756360 2.4.1.10 440 - sucrose transfructosylating activity, at pH 6.0 and 37°C 756360 2.4.1.10 467 - sucrose transfructosylating activity, at pH 6.0 and 37°C 756360 2.4.1.10 479 - sucrose transfructosylating activity, at pH 6.0 and 37°C 756360 2.4.1.10 480 - sucrose total activity, at pH 6.0 and 37°C 756360 2.4.1.10 480.4 - sucrose pH 6.6, mutant enzyme W172A 678072 2.4.1.10 519 - sucrose transfructosylating activity, at pH 6.0 and 37°C 756360 2.4.1.10 617 - sucrose hydrolytic activity, at pH 6.0 and 37°C 756360 2.4.1.10 674 - sucrose transfructosylating activity, at pH 6.0 and 37°C 756360 2.4.1.10 795.22 - sucrose mutant enzyme N251W/K372Y, total activity, at pH 6.0 and 50°C 756958 2.4.1.10 820.75 - sucrose mutant enzyme N251W, hydrolytic activity, at pH 6.0 and 50°C 756958 2.4.1.10 978.37 - sucrose mutant enzyme N251W, total activity, at pH 6.0 and 50°C 756958