1.1.1.45 additional information - additional information kinetic studies 286905, 286907 1.1.1.45 additional information - additional information no activity with 5-40 mM sugars or hydroxyacids like 2-keto-L-guluonate, 3-deoxyglucosone, xylitol, L-threitol, ascorbic acid, malic acid, L-threonine, L-glycerate, L-tartarate and L-lactate, or 50 mM ethyl and methyl esters of 3-hydroxybutyrate 669147 1.1.1.45 0.0005 - NADH at pH 8.5, rabbit liver GDH 669147 1.1.1.45 0.0006 - NADH at pH 8.5, recombinant rabbit GDH 669147 1.1.1.45 0.01 - NAD+ at pH 8.5, recombinant rabbit GDH 669147 1.1.1.45 0.01 - NAD+ wild-type enzyme, pH 7.0, 25°C 712765 1.1.1.45 0.011 - NAD+ at pH 8.5, rabbit liver GDH 669147 1.1.1.45 0.018 - L-gulonate with NAD+, wild-type enzyme, pH 7.0, 25°C 712765 1.1.1.45 0.028 - NAD+ mutant enzyme Q41N, pH 7.0, 25°C 712765 1.1.1.45 0.04 - NAD+ cosubstrate L-gulonate, pH 8.5 286905