1.1.99.35	additional information	-	additional information	under conditions that avoid its masking by sugar-substrate inhibition as much as possible, negative kinetic cooperativity with respect to sugar substrate oxidation is observed. The cooperativity effect dramatically changes the performance of soluble GDH, as reflected by the V2 and K2 values for glucose in phosphate buffer being about 10-fold and 100-fold higher than the V1 and K1 values, respectively. Substituting the Ca2+ involved in activation of pyrroloquinoline quinone in soluble GDH by Sr2+ affects the cooperativity effect but not the two turnover rates of the hybrid enzyme for glucose	703532	
1.1.99.35	0.0014	-	lactose	electron transfer mediator: 1-(N,N-dimethylamine)-4-(4-morpholine)benzene, pH 8.0, temperature not specified in the publication	762923	
1.1.99.35	0.0018	-	lactose	electron transfer mediator: 1-(N,N-dimethylamine)-4-(4-morpholine)benzene, pH 7.0, temperature not specified in the publication	762923	
1.1.99.35	0.0019	-	lactose	electron transfer mediator: N,N'-dimethyl-4,4'-azopyridinium methyl sulfate, pH 8.0, temperature not specified in the publication	762923	
1.1.99.35	0.0023	-	lactose	electron transfer mediator: N,N'-dimethyl-4,4'-azopyridinium methyl sulfate, pH 7.0, temperature not specified in the publication	762923	
1.1.99.35	0.74	-	D-glucose	pH and temperature not specified in the publication	762834	
1.1.99.35	2	3	3-O-methyl-D-glucose	recombinant wild-type with R4-tail, pH 7.0	655929	
1.1.99.35	2	3	D-glucose	recombinant wild-type with R4-tail, pH 7.0	655929	
1.1.99.35	2.7	-	D-galactose	mutant N462H, pH 7.0	654398	
1.1.99.35	3.7	-	D-galactose	mutant N452T, pH 7.0	654398