1.7.1.B2	2,4,6-trinitrotoluene + NADH + H+	-	
1.7.1.B2	2,4,6-trinitrotoluene + NADPH + H+	-	
1.7.1.B2	2,4-dinitrotoluene + NADPH + H+	-	
1.7.1.B2	2,4-dinitrotoluene + NADPH + H+	highest activity	
1.7.1.B2	2,6-dinitrotoluene + NADPH + H+	-	
1.7.1.B2	2,6-dinitrotoluene + NADPH + H+	highest activity	
1.7.1.B2	additional information	Escherichia coli nitroreductase NfsB can reduce nitrochloromethylbenzindolines, i.e. nitro-CBIs, in an oxygen-independent fashion, generating highly cytotoxic metabolites that alkylate the N3 of adenine in the minor groove of DNA	
1.7.1.B2	additional information	Pseudomonas aeruginosa nitroreductase NfsB can reduce nitrochloromethylbenzindolines, i.e. nitro-CBIs, in an oxygen-independent fashion, generating highly cytotoxic metabolites that alkylate the N3 of adenine in the minor groove of DNA