1.3.1.109 (E)-but-2-enoyl-CoA + 2 NADH + 2 oxidized ferredoxin iron-sulfur cluster - 1.3.1.109 (E)-but-2-enoyl-CoA + 2 NADH + 2 oxidized ferredoxin iron-sulfur cluster the energy-rich reduced ferredoxin contributes to the energy conservation of the organism either by regeneration of NADH via the H+/Na+-pumping ferredoxin-NAD+ reductase also (Rnf) or by reduction of protons to H2, which increases the substrate-level phosphorylation via the oxidative branch of the fermentation 1.3.1.109 (E)-but-2-enoyl-CoA + NADH + oxidized ferredoxin [iron-sulfur] cluster - 1.3.1.109 butanoyl-CoA + 2 NAD+ + 2 reduced ferredoxin [iron-sulfur] cluster -