2.3.1.84	analysis	method to quantify the activity of alcohol acetyltransferase using a SnO2-based sensor of electronic nose, on the basis of its higher sensitivity to the reducing alcohol than the oxidizing ester. The method has been successfully applied to determine the alcohol acetyltransferase activity of a cider yeast strain	
2.3.1.84	biotechnology	a low-cost fermentation process for isoamyl acetate biosynthesis by overexpressing the yeast alcohol acetyl-transferase AFT1 in Escherichia coli is developed	
2.3.1.84	biotechnology	a low-cost fermentation process for isoamyl acetate biosynthesis by overexpressing the yeast alcohol acetyl-transferase AFT2 in Escherichia coli is developed	
2.3.1.84	food industry	after 5 days of fermentation, the concentrations of ethyl acetate, isoamyl acetate, and isobutyl acetate in yellow rice wines fermented with yeasts overexpressing isoform ATF2 increase to 137.79 mg/L (an approximate 4.9fold increase relative to the parent cell), 26.68 mg/L, and 7.60 mg/L, respectively	
2.3.1.84	nutrition	polishing of rice for use in sake brewing is necessary to remove inositol from rice, thereby increasing AATase activity. A high AATase activity leads to an abundance of acetate esters of higher alcohols in sake, such as isoamyl acetate, one of the most favorable odor-enhancing compounds	
2.3.1.84	synthesis	engineering of Escherichia coli to produce biodiesel, which depends on the expression of Saccharomyces cerevisiae alcohol acetyltransferase ATF1, fatty acyl-ACP reductase from Synechococcus elongates, carboxylic acid reductase from Mycobacterium marinum, fatty acyl-CoA reductase from Marinobacter aquaeolei, aldehyde reductase from Escherichia coli, fatty acyl-CoA synthetase from Escherichia coli, and TesA, a truncated fatty acyl-ACP thioesterase from Escherichia coli	
2.3.1.84	synthesis	overexpression of ATF1 in Chinese liquor yeast through precise and seamless insertion of PGK1 promoter. In the liquid fermentation of corn hydrolysate, both mRNA level and alcohol acetyltransferase activity of ATF1 in mutant are pronounced higher than the parental strain. Productivity of ethyl acetate increases from 25.04 to 78.76 mg/l	
2.3.1.84	synthesis	production of acetate esters by a mutant alpha5 strain overexpressing ATF1 with aminotransferase BAT2 deletion is 1353.4 mg/l, which is 43.16fold higher than that by the original strain. Isoform ATF1 overexpression with BAT2 deletion can further increase the ratio of ester to higher alcohol to a greater extent than isoform ATF2 overexpression with BAT2 deletion	
2.3.1.84	synthesis	production of acetate esters by a mutant alpha5 strain overexpressing ATF1 with aminotransferase BAT2 deletion is 73.4 mg/l, which is 2.34fold higher than that by the original strain. Isoform ATF1 overexpression with BAT2 deletion can further increase the ratio of ester to higher alcohol to a greater extent than isoform ATF2 overexpression with BAT2 deletion