2.3.1.84 - 487558, 487565, 676539, 758549 2.3.1.84 ammonium sulfate, Biofine HIC-PH, DEAE-5PW, Mono-P 487556 2.3.1.84 ammonium sulfate, Sephadex G-150, DEAE-Sephadex A-50 487557 2.3.1.84 enzyme is solubilized from lipid particles using 1% Triton X-100 and then purified by three steps of chromatographic separations with diethylaminoethlyl (DEAE) Sepharose, Sephadex G-75 and Octyl Sepharose, respectively 688115 2.3.1.84 partially purified 487561 2.3.1.84 PBE 94 column, DEAE-cellulose, Toypearl HW60, hydroxyapatite, octyl Sepharose 487559 2.3.1.84 recombinant protein purified to 99% homogeneity using nickel-based affinity chromatography 723419 2.3.1.84 recombinant protein, partial 720138 2.3.1.84 recombinant renzyme, partial 719627 2.3.1.84 Sepharose 6B, DEAE-Sephadex A50, partially purified 487555