3.4.24.28 - 31159, 31163 3.4.24.28 after expression in Escherichia coli with N-terminal His-tag 670771 3.4.24.28 extracellular enzyme from culture medium by anion exchange chromatography 718012 3.4.24.28 extracellular enzyme from culture medium by several chromatographic steps including one affinity based step 718012 3.4.24.28 native enzyme 13.1fold by ethanol precipitation, gel filtration, and anion exchange chromatography 733469 3.4.24.28 native extracellular enzyme 5.6fold from culture supernatant by ammonium sulfate fractionation, anion exchange and hydrophobic interaction chromatography, and gel filtration 733130 3.4.24.28 recombinant enzyme 701210 3.4.24.28 recombinant enzyme from Bacillus subtilis culture medium by ammonium sulfate fractionation and hydrophobic interaction chromatography to homogeneity 717233 3.4.24.28 recombinant enzyme from Escherichia coli strain BL21(DE3) 733167 3.4.24.28 wild-type and mutant enzymes 651101 3.4.24.28 wild-type and mutant TLPs 650655