1.3.1.70 - 288491, 288492 1.3.1.70 partial 288489, 288495 1.3.1.70 recombinant His8-tagged, seleniummethionine-labeled wild-type and mutant enzymes from Escherichia coli strain C43(DE3) by nickel affinity chromatography and gel filtration 745882 1.3.1.70 Sephadex columns to de-salt enzyme preparation: 5fold activation 288489 1.3.1.70 solubilization with combined octylglucoside and sodium taurodeoxycholate system and partial purification 288490