1.7.1.B2	His-bind Ni-NTA column chromatography	
1.7.1.B2	Ni-NTA column chromatography	
1.7.1.B2	Ni-NTA resin column chromatography	
1.7.1.B2	recombinant wild-type and mutant N-terminally His6-tagged enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography to over 95% purity