1.1.1.173 - 1.1.1.173 native enzyme 42.8fold by anion exchange chromatography, ultrafiltration, gel filtration, hydroxyapatite chromatography, and again gel filtration, recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography 1.1.1.173 recombinant enzyme in analytical amounts from Saccharomyces cerevisiae by anion exchange chromatography, and native and SDS-PAGE, tagging of the enzyme strongly affects its activity, overview 1.1.1.173 recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography