1.8.99.2	-	
1.8.99.2	DE-52 column chromatography, macro-DEAE column chromatography, and hydroxyapatite column chromatography	
1.8.99.2	DEAE-Biogel A chromatography, Source 15 column chromatography, and Superdex 200 column chromatography	
1.8.99.2	DEAE-Toyopearl 650S column chromatography, Sephacryl S-200 gel filtration, and Superdex 200 gel filtration	
1.8.99.2	G100A	
1.8.99.2	HiTrap chelating column chromatography	
1.8.99.2	isolated under the exclusion of oxygen	
1.8.99.2	Ni2+ affinity chromatography	
1.8.99.2	Ni2+-affinity column chromatography	
1.8.99.2	partial purification by ammonium sulfate precipitation and Q-Sepharose column chromatography