4.6.1.24 - 4.6.1.24 anion-exchange chromatography and gel filtration 4.6.1.24 diethylaminoethylcellulose chromatography 4.6.1.24 fusion protein of the antiferritin antibody VL domain and barnase 4.6.1.24 gel filtration 4.6.1.24 ion-exchange chromatography and gel filtration 4.6.1.24 on porous glass affinity adsorbent 4.6.1.24 recombinant enzyme from Escherichia coli strain BL21(DE3)pLysS to homogeneity by ammonium sulfate fractionation, gel filtration, cation exchange and anion exchange chromatography, and again gel filtration, heparin affinity chromatography, and dialysis 4.6.1.24 recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) pLysS to homogeneity by ammonium sulfate fractionation, gel filtration, cation exchange and anion exchange chromatography, and again gel filtration, heparin affinity chromatography, and dialysis 4.6.1.24 RNase T1 variants E46N and Y45W/E46N