6.3.4.3 ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate direct formylation at the N-10 position, solvent oxygen is not incorporated into N10-formyltetrahydrofolate 1521 6.3.4.3 ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate sequential mechanism, formyl phosphate is an intermediate 1526 6.3.4.3 ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate stepwise mechanism involving a dissociable intermediate, consistent only with a sequential mechanism 1509 6.3.4.3 ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate stepwise reaction mechanism, formyl phosphate is a potential intermediate 1511 6.3.4.3 ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate substrate binds to the enzyme in a random fashion, products are not released until all substrates are bound 1506 6.3.4.3 ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate the enzyme is not trifunctional showing only 10-formyl-THS synthetase activity, enzyme evolutionary considerations 660817