2.4.1.10 cell culture enzyme production is performed by free and differently immobilized cells, maximum yield with 18.47 U/ml is achieved after 48 h in batch shaken culture of the free cells, but immobilized culture technique allows the achievement of relatively high enzyme activities of 5.5-10.75 U/ml with successful repeated utilization of the immobilized cells 701566 2.4.1.10 commercial preparation - 736648 2.4.1.10 culture medium - -, 671449 2.4.1.10 culture supernatant - -, 488342, 678072 2.4.1.10 culture supernatant ethanol precipitation 689722 2.4.1.10 kernel - 736970 2.4.1.10 kernel developing from anthesis until maturity. 1,6-Kestotetraose formation is prominent during the first 13 days after anthesis, and little or no 6-SFT activity is observed later on during maturation 736970 2.4.1.10 leaf - 488334 2.4.1.10 leaf blade the relative expression of the fructan biosynthetic genes 1-SST and 6-SFT, encoding sucrose:sucrose 1-fructosyltransferase and sucrose:fructan 6-fructosyltransferase, respectively, and 6G-FFT/1-FFT isoform 6G-FFT 2 correlates positively with the accumulation of fructans in both blades and pseudostems. Isoform 6G-FFT 2 is highly expressed in cultivar E, that accumulatss the highest levels of fructans in this study 736695 2.4.1.10 planktonic cell expression of the levan-forming enzyme, levansucrase, occurrs mainly during early exponential growth of both planktonic and sessile cells 675845