1.13.11.27 additional information - activity of 0.0033 micromol/min/mg for 2YT-grown Escherichia coli DH5alpha extract and 0.0018 micromol/min/mg for LB-grown Escherichia coli DH5alpha 677278 1.13.11.27 additional information - methods for assay 395389 1.13.11.27 additional information - the oxygen electrode provides an excellent measure of enzyme activity under conditions where maximum enzyme activity is obtainable in the absence of a reducing agent 395374 1.13.11.27 additional information - with substrate 2,3,5,6-tetraproteo 4-hydroxyphenylpyruvate and the solvent H2O Vmax = 1.9/s, with substrate 2,3,5,6-tetradeutero 4-hydroxyphenylpyruvate and the solvent H2O Vmax = 1.8/s, with substrate 2,3,5,6-tetraproteo 4-hydroxyphenylpyruvate and the solvent D2O Vmax = 0.86/s 672033 1.13.11.27 0.004 - protein expressed in Escherichia coli DH5alpha 677278 1.13.11.27 0.04 - purified recombinant mutant R378K, pH 6.5, 37°C, homogentisate production 726347 1.13.11.27 0.06 - purified recombinant mutant E254D, pH 6.5, 37°C, O2 consumption 726347 1.13.11.27 0.07 - purified recombinant mutant R378K, pH 6.5, 37°C, O2 consumption 726347 1.13.11.27 0.11 0.2 protein expressed in Escherichia coli SG13009 677278 1.13.11.27 0.17 0.26 protein expressed in Escherichia coli M15(pRep4) 677278 1.13.11.27 0.2 - purified recombinant mutant E254D, pH 6.5, 37°C, homogentisate production 726347 1.13.11.27 0.51 1.1 enzyme forms 1, 2, and 3 395382 1.13.11.27 0.53 - - 395381 1.13.11.27 0.6 0.9 - 395387 1.13.11.27 0.92 - - 395384 1.13.11.27 1 - - 395369 1.13.11.27 2.33 - - 395396 1.13.11.27 2.6 - purified recombinant wild-type enzyme, pH 6.5, 37°C, O2 consumption 726347 1.13.11.27 2.8 - purified recombinant wild-type enzyme, pH 6.5, 37°C, homogentisate production 726347 1.13.11.27 8.5 - - 395370, 395386 1.13.11.27 10.6 - pH 7.0, 25°C 657978 1.13.11.27 39.6 - - 395377 1.13.11.27 51 - - 395367