1.1.1.391 0.35 - pH 9.5, 25°C, substrate 3,7-dioxo-5beta-cholan-24-oic acid 286228 1.1.1.391 0.38 - pH 9.5, 25°C, substrate 7alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid 286228 1.1.1.391 0.59 - pH 9.5, 25°C, substrate 3,12-dioxo-5beta-cholan-24-oic acid 286228 1.1.1.391 1 - pH 9.5, 25°C, substrate 7alpha,12alpha-3-oxo-dihydroxy-5beta-cholan-24-oic acid 286228 1.1.1.391 2.12 - pH 9.5, 25°C, substrate 3,7,12-trioxo-5beta-cholan-24-oic acid 286228 1.1.1.391 2.35 - pH 9.5, 25°C, substrate 3,7-dioxo-5beta-cholan-24-oic acid 286228 1.1.1.391 2.45 - pH 9.5, 25°C, substrate 3beta,12alpha-dihydroxy-5beta-cholan-24-oic acid 286228 1.1.1.391 2.49 - pH 9.5, 25°C, substrate 12alpha-hydroxy-3-oxo-5beta-cholate 286228 1.1.1.391 3.2 - purified enzyme, pH 10.0-10.2, 37°C 735471