1.3.1.31 -18°C, pH 7, buffer containing 10 mM phosphate and 0.1 M KCl, formation of a dissociation product sedimenting at about 10 S 1.3.1.31 -20°C, phosphate buffer, pH 7.0, containing (E)-2-methyl-2-butenoate, EDTA, sucrose and 2-mercaptoethanol, repeatedly thawed and frozen, 50% loss of activity 1.3.1.31 4°C, anaerobic conditions, no loss of activity