1.1.99.35 2-deoxy-D-glucose + oxidized 2,6-dichlorophenolindophenol - Acinetobacter calcoaceticus 2-deoxy-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol - ? 407848 1.1.99.35 2-deoxy-D-glucose + oxidized 2,6-dichlorophenolindophenol 4% of the activity with D-glucose Acinetobacter calcoaceticus 2-deoxy-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol - ? 407848 1.1.99.35 3-O-methyl-D-glucose + oxidized 2,6-dichlorophenolindophenol - Acinetobacter calcoaceticus 3-O-methyl-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol - ? 408033 1.1.99.35 3-O-methyl-D-glucose + oxidized 2,6-dichlorophenolindophenol 81% of the activity with D-glucose Acinetobacter calcoaceticus 3-O-methyl-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol - ? 408033 1.1.99.35 cellobiose + N-methylphenazonium methylsulfate - Acinetobacter calcoaceticus cellobiono-1,5-lactone + reduced N-methylphenazonium methylsulfate - ? 439295 1.1.99.35 cellobiose + oxidized 2,6-dichlorophenolindophenol - Acinetobacter calcoaceticus ? + reduced 2,6-dichlorophenolindophenol - ? 408829 1.1.99.35 D-allose + N-methylphenazonium methylsulfate - Acinetobacter calcoaceticus D-allono-1,5-lactone + reduced N-methylphenazonium methylsulfate - ? 439376 1.1.99.35 D-allose + oxidized 2,6-dichlorophenolindophenol - Acinetobacter calcoaceticus D-allono-1,5-lactone + reduced 2,6-dichlorophenolindophenol - ? 409127 1.1.99.35 D-allose + oxidized 2,6-dichlorophenolindophenol 47% of the activity with D-glucose Acinetobacter calcoaceticus D-allono-1,5-lactone + reduced 2,6-dichlorophenolindophenol - ? 409127 1.1.99.35 D-fucose + oxidized 2,6-dichlorophenolindophenol - Acinetobacter calcoaceticus D-fucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol - ? 409141 1.1.99.35 D-galactose + N-methylphenazonium methylsulfate 30% activity compared to D-glucose Acinetobacter calcoaceticus D-galactono-1,5-lactone + reduced N-methylphenazonium methylsulfate - ? 439401 1.1.99.35 D-galactose + oxidized 2,6-dichlorophenolindophenol - Acinetobacter calcoaceticus D-galactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol - ? 409143 1.1.99.35 D-galactose + oxidized 2,6-dichlorophenolindophenol 11% of the activity with D-glucose Acinetobacter calcoaceticus D-galactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol - ? 409143 1.1.99.35 D-galactose + oxidized 2,6-dichlorophenolindophenol about 13% activity compared to D-glucose Acinetobacter calcoaceticus D-galactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol - ? 409143 1.1.99.35 D-galactose + oxidized 2,6-dichlorophenolindophenol about 13% activity compared to D-glucose Acinetobacter calcoaceticus L.M.D. 79.41 D-galactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol - ? 409143 1.1.99.35 D-galactose + oxidized N-methylphenazonium methyl sulfate - Acinetobacter calcoaceticus D-galactono-1,5-lactone + reduced N-methylphenazonium methyl sulfate - ? 355898 1.1.99.35 D-glucose + 2,6-dichlorophenolindophenol - Pseudomonas putida D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol - ? 404823 1.1.99.35 D-glucose + 2,6-dichlorophenolindophenol - Serratia marcescens D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol - ? 404823 1.1.99.35 D-glucose + 2,6-dichlorophenolindophenol - Pseudomonas putida KT 2240 D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol - ? 404823 1.1.99.35 D-glucose + 2,6-dichlorophenolindophenol - Serratia marcescens CTM 50650 D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol - ? 404823 1.1.99.35 D-glucose + acceptor - Acinetobacter calcoaceticus D-glucono-1,5-lactone + reduced acceptor - ? 386427 1.1.99.35 D-glucose + dimethyl((6-methyl-7-((4-nitrosophenyl)amino)-1H-imidazo[1,2-b]pyrazol-1-yl)methyl)phosphine oxide - Acinetobacter calcoaceticus D-glucono-1,5-lactone + reduced dimethyl((6-methyl-7-((4-nitrosophenyl)amino)-1H-imidazo[1,2-b]pyrazol-1-yl)methyl)phosphine oxide - ? 460936 1.1.99.35 D-glucose + N-methylphenazonium methylsulfate 100% activity Acinetobacter calcoaceticus D-glucono-1,5-lactone + reduced N-methylphenazonium methylsulfate - ? 439420 1.1.99.35 D-glucose + osmium polymer - Acinetobacter calcoaceticus D-glucono-1,5-lactone + reduced osmium polymer - ? 439422 1.1.99.35 D-glucose + oxidized 2,6-dichlorophenol-indophenol - Acinetobacter calcoaceticus D-glucono-1,5-lactone + reduced 2,6-dichlorophenol-indophenol - ? 409146 1.1.99.35 D-glucose + oxidized 2,6-dichlorophenolindophenol - Acinetobacter calcoaceticus D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol - ? 409147 1.1.99.35 D-glucose + oxidized 2,6-dichlorophenolindophenol 100% activity Acinetobacter calcoaceticus D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol - ? 409147 1.1.99.35 D-glucose + oxidized 2,6-dichlorophenolindophenol 100% activity Acinetobacter calcoaceticus L.M.D. 79.41 D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol - ? 409147 1.1.99.35 D-glucose + oxidized N-methylphenazonium methyl sulfate - Acinetobacter calcoaceticus D-glucono-1,5-lactone + reduced N-methylphenazonium methyl sulfate - ? 355890 1.1.99.35 D-glucose + phenazine methosulfate - Pseudomonas putida D-glucono-1,5-lactone + reduced phenazine methosulfate - ? 231182 1.1.99.35 D-glucose + phenazine methosulfate - Serratia marcescens D-glucono-1,5-lactone + reduced phenazine methosulfate - ? 231182 1.1.99.35 D-glucose + phenazine methosulfate - Pseudomonas putida KT 2240 D-glucono-1,5-lactone + reduced phenazine methosulfate - ? 231182 1.1.99.35 D-glucose + phenazine methosulfate - Serratia marcescens CTM 50650 D-glucono-1,5-lactone + reduced phenazine methosulfate - ? 231182 1.1.99.35 D-lyxose + N-methylphenazonium methylsulfate - Acinetobacter calcoaceticus D-lyxono-1,5-lactone + reduced N-methylphenazonium methylsulfate - ? 439440 1.1.99.35 D-lyxose + oxidized 2,6-dichlorophenolindophenol - Acinetobacter calcoaceticus D-lyxono-1,5-lactone + reduced 2,6-dichlorophenolindophenol - ? 409161 1.1.99.35 D-mannose + N-methylphenazonium methylsulfate - Acinetobacter calcoaceticus D-mannono-1,5-lactone + reduced N-methylphenazonium methylsulfate - ? 439446 1.1.99.35 D-mannose + oxidized 2,6-dichlorophenolindophenol - Acinetobacter calcoaceticus D-mannono-1,5-lactone + reduced 2,6-dichlorophenolindophenol - ? 409162 1.1.99.35 D-mannose + oxidized 2,6-dichlorophenolindophenol 13% of the activity with D-glucose Acinetobacter calcoaceticus D-mannono-1,5-lactone + reduced 2,6-dichlorophenolindophenol - ? 409162 1.1.99.35 D-mannose + oxidized 2,6-dichlorophenolindophenol about 11% activity compared to D-glucose Acinetobacter calcoaceticus D-mannono-1,5-lactone + reduced 2,6-dichlorophenolindophenol - ? 409162 1.1.99.35 D-ribose + N-methylphenazonium methylsulfate 5% activity compared to D-glucose Acinetobacter calcoaceticus D-ribono-1,5-lactone + reduced N-methylphenazonium methylsulfate - ? 439458 1.1.99.35 D-ribose + oxidized 2,6-dichlorophenolindophenol - Acinetobacter calcoaceticus ? + reduced 2,6-dichlorophenolindophenol - ? 409172 1.1.99.35 D-xylose + N-methylphenazonium methylsulfate 14% activity compared to D-glucose Acinetobacter calcoaceticus D-xylono-1,5-lactone + reduced N-methylphenazonium methylsulfate - ? 439473 1.1.99.35 D-xylose + oxidized 2,6-dichlorophenolindophenol - Acinetobacter calcoaceticus D-xylono-1,5-lactone + reduced 2,6-dichlorophenolindophenol - ? 409181 1.1.99.35 D-xylose + oxidized 2,6-dichlorophenolindophenol 7% of the activity with D-glucose Acinetobacter calcoaceticus D-xylono-1,5-lactone + reduced 2,6-dichlorophenolindophenol - ? 409181 1.1.99.35 D-xylose + oxidized 2,6-dichlorophenolindophenol about 9% activity compared to D-glucose Acinetobacter calcoaceticus D-xylono-1,5-lactone + reduced 2,6-dichlorophenolindophenol - ? 409181 1.1.99.35 D-xylose + oxidized N-methylphenazonium methyl sulfate - Acinetobacter calcoaceticus D-xylono-1,5-lactone + reduced N-methylphenazonium methyl sulfate - ? 355892 1.1.99.35 L-arabinose + N-methylphenazonium methylsulfate - Acinetobacter calcoaceticus L-arabinono-1,5-lactone + reduced N-methylphenazonium methylsulfate - ? 439643 1.1.99.35 L-arabinose + oxidized 2,6-dichlorophenolindophenol - Acinetobacter calcoaceticus L-arabinono-1,5-lactone + reduced 2,6-dichlorophenolindophenol - ? 409625 1.1.99.35 lactose + 1-(N,N-dimethylamine)-4-(4-morpholine)benzene - Acinetobacter calcoaceticus lactono-1,5-lactone + reduced 1-(N,N-dimethylamine)-4-(4-morpholine)benzene - ? 461194 1.1.99.35 lactose + N,N'-dimethyl-4,4'-azopyridinium methyl sulfate - Acinetobacter calcoaceticus lactono-1,5-lactone + reduced N,N'-dimethyl-4,4'-azopyridinium methyl sulfate - ? 461197 1.1.99.35 lactose + N-methylphenazonium methylsulfate 4% activity compared to D-glucose Acinetobacter calcoaceticus lactono-1,5-lactone + reduced N-methylphenazonium methylsulfate - ? 439706 1.1.99.35 lactose + oxidized 2,6-dichlorophenol-indophenol - Acinetobacter calcoaceticus ? + reduced 2,6-dichlorophenol-indophenol - ? 409738 1.1.99.35 lactose + oxidized 2,6-dichlorophenolindophenol - Acinetobacter calcoaceticus ? + reduced 2,6-dichlorophenolindophenol - ? 409739 1.1.99.35 lactose + oxidized 2,6-dichlorophenolindophenol 61% of the activity with D-glucose Acinetobacter calcoaceticus ? + reduced 2,6-dichlorophenolindophenol - ? 409739 1.1.99.35 lactose + oxidized 2,6-dichlorophenolindophenol - Acinetobacter calcoaceticus lactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol - ? 409740 1.1.99.35 lactose + oxidized 2,6-dichlorophenolindophenol 61% of the activity with D-glucose Acinetobacter calcoaceticus lactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol - ? 409740 1.1.99.35 lactose + oxidized 2,6-dichlorophenolindophenol about 50% activity compared to D-glucose Acinetobacter calcoaceticus lactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol - ? 409740 1.1.99.35 lactose + oxidized N-methylphenazonium methyl sulfate - Acinetobacter calcoaceticus 4-O-beta-D-galactopyranosyl-D-glucono-1,5-lactone + reduced N-methylphenazonium methyl sulfate - ? 409741 1.1.99.35 maltose + acceptor - Acinetobacter calcoaceticus maltono-1,5-lactone + reduced acceptor - ? 426635 1.1.99.35 maltose + N-methylphenazonium methylsulfate 12% activity compared to D-glucose Acinetobacter calcoaceticus maltono-1,5-lactone + reduced N-methylphenazonium methylsulfate - ? 439727 1.1.99.35 maltose + oxidized 2,6-dichlorophenolindophenol - Acinetobacter calcoaceticus ? + reduced 2,6-dichlorophenolindophenol - ? 409842 1.1.99.35 maltose + oxidized 2,6-dichlorophenolindophenol 61% of the activity with D-glucose Acinetobacter calcoaceticus ? + reduced 2,6-dichlorophenolindophenol - ? 409842 1.1.99.35 maltose + oxidized 2,6-dichlorophenolindophenol - Acinetobacter calcoaceticus maltono-1,5-lactone + reduced 2,6-dichlorophenolindophenol - ? 409843 1.1.99.35 maltose + oxidized 2,6-dichlorophenolindophenol 61% of the activity with D-glucose Acinetobacter calcoaceticus maltono-1,5-lactone + reduced 2,6-dichlorophenolindophenol - ? 409843 1.1.99.35 maltose + oxidized 2,6-dichlorophenolindophenol about 70% activity compared to D-glucose Acinetobacter calcoaceticus maltono-1,5-lactone + reduced 2,6-dichlorophenolindophenol - ? 409843 1.1.99.35 melibiose + N-methylphenazonium methylsulfate 5% activity compared to D-glucose Acinetobacter calcoaceticus melibiono-1,5-lactone + reduced N-methylphenazonium methylsulfate - ? 439737 1.1.99.35 melibiose + oxidized 2,6-dichlorophenolindophenol - Acinetobacter calcoaceticus ? + reduced 2,6-dichlorophenolindophenol - ? 409859 1.1.99.35 additional information kinetic mechanism of sGDH consists of (a) step(s) in which a fluorescing intermediate is formed, and a subsequent, irreversible step, determining the overall rate of the reductive half-reaction. The pH optimum determined for glucose oxidation appears to be 7.0, implying that reoxidation of sGDHred is rate-limiting with those electron acceptors displaying a different value under steady-state conditions. Acinetobacter calcoaceticus ? - ? 89 1.1.99.35 additional information mechanism comprises general base-catalyzed hydride transfer Acinetobacter calcoaceticus ? - ? 89 1.1.99.35 additional information the sequential steps in the mechanism of sGDH must be reversible substrate binding, direct transfer of a hydride ion (reversible or irreversible) from the C1 position of the beta-anomer of glucose to the C5 of PQQ, irreversible, rate-determining tautomerization of the fluorescing, C5-reduced PQQ to PQQH2 and release (or earlier) of the product, D-glucono-delta-lactone, and oxidation of PQQH2 by an electron acceptor. The PQQ-activating Ca2+ greatly facilitates the reactions occurring in the second step. His144 may also play a role in this by acting as a general base catalyst, initiating hydride transfer by abstracting a proton from the anomeric OH group of glucose Acinetobacter calcoaceticus ? - ? 89 1.1.99.35 additional information almost no activity with D-fructose Acinetobacter calcoaceticus ? - ? 89 1.1.99.35 additional information no activity with D-glucosamine, L-altrose, L-lyxose, L-talose, L-mannose, D-altrose, D-arabinose, D-talose, L-xylose, L-glucose, L-galactose, L-allose, sucrose, trehalose, and raffinose Acinetobacter calcoaceticus ? - ? 89 1.1.99.35 additional information almost no activity with D-fructose Acinetobacter calcoaceticus L.M.D. 79.41 ? - ? 89