1.2.1.99	2-furaldehyde + NAD+ + H2O	22% of the activity with 3-hydroxypropionaldehyde	Klebsiella pneumoniae	furan-2-carboxylate + NADH + 2 H+	-	?	413941	
1.2.1.99	3-hydroxypropionaldehyde + NAD+ + H2O	-	Klebsiella pneumoniae	3-hydroxypropanoate + NADH + 2 H+	-	?	399543	
1.2.1.99	3-hydroxypropionaldehyde + NAD+ + H2O	-	Escherichia coli	3-hydroxypropanoate + NADH + 2 H+	-	?	399543	
1.2.1.99	3-hydroxypropionaldehyde + NAD+ + H2O	the enzyme might play a role in putresine and propionate metabolism	Klebsiella pneumoniae	3-hydroxypropanoate + NADH + 2 H+	-	?	399543	
1.2.1.99	3-hydroxypropionaldehyde + NAD+ + H2O	activity for the reduction of 3-hydroxypropionate to 3-hydroxypropionaldehyde is only 4% of that for the corresponding oxidation reaction, indicating that this enzyme catalyzes oxidation reactions under most physiological conditions	Escherichia coli	3-hydroxypropanoate + NADH + 2 H+	-	r	399543	
1.2.1.99	3-hydroxypropionaldehyde + NAD+ + H2O	3-hydroxypropionaldehyde i.e. 3-HPA, 3-hydroxypropionic acid i.e. 3-HP, PuuC overexpression enhances the glycerol carbon flux towards 3-hydroxypropionaldehyde	Klebsiella pneumoniae	3-hydroxypropanoate + NADH + 2 H+	-	?	399543	
1.2.1.99	3-hydroxypropionaldehyde + NAD+ + H2O	-	Klebsiella pneumoniae DSM 2026	3-hydroxypropanoate + NADH + 2 H+	-	?	399543	
1.2.1.99	3-hydroxypropionaldehyde + NADP+ + H2O	activity is decreased by 73% when NAD+ is replaced with NADP+. The reduction of 3-hydroxypropionate to 3-hydroxypropionaldehyde is not catalyzed with NADPH as cofactor	Escherichia coli	3-hydroxypropanoate + NADPH + 2 H+	-	ir	414043	
1.2.1.99	4-(gamma-L-glutamylamino)butanal + NAD+ + H2O	-	Escherichia coli	4-(gamma-L-glutamylamino)butanoate + NADH + H+	-	?	438822	
1.2.1.99	acetaldehyde + NAD+ + H2O	-	Klebsiella pneumoniae	acetate + NADH + H+	-	?	189125	
1.2.1.99	acetaldehyde + NAD+ + H2O	-	Escherichia coli	acetate + NADH + H+	-	?	189125	
1.2.1.99	benzaldehyde + NAD+ + H2O	-	Escherichia coli	benzoate + NADH + H+	-	?	189127	
1.2.1.99	benzaldehyde + NAD+ + H2O	35% of the activity with 3-hydroxypropionaldehyde	Klebsiella pneumoniae	benzoate + NADH + H+	-	?	189127	
1.2.1.99	butyraldehyde + NAD+ + H2O	-	Klebsiella pneumoniae	butyrate + NADH + 2 H+	-	?	414401	
1.2.1.99	butyraldehyde + NAD+ + H2O	-	Escherichia coli	butyrate + NADH + 2 H+	-	?	414401	
1.2.1.99	gamma-glutamyl-gamma-aminobutyraldehyde + NAD+ + H2O	the enzyme is involved in degradation of putrescine	Escherichia coli	4-(glutamylamino) butanoate + NADH + 2 H+	-	?	413387	
1.2.1.99	isovaleraldehyde + NAD+ + H2O	-	Klebsiella pneumoniae	3-methylbutanoate + NADH + 2 H+	-	?	414733	
1.2.1.99	isovaleraldehyde + NAD+ + H2O	-	Escherichia coli	3-methylbutanoate + NADH + 2 H+	-	?	414733	
1.2.1.99	additional information	no activity with formaldehyde	Klebsiella pneumoniae	?	-	?	89	
1.2.1.99	additional information	development and evaluation recombinant Klebsiella pneumoniae, for the production of 3-hydroxypropionic acid from glycerol, therefor PuuC together with a coenzyme B12-dependent glycerol dehydratase are overexpressed while two major oxidoreductases, DhaT and YqhD, are disrupted eliminating the production of 1,3-propanediol out of 3-hydroxypropionaldehyde	Klebsiella pneumoniae	?	-	?	89	
1.2.1.99	additional information	enzyme additionally displays the activity of EC 1.2.1.24, succinate-semialdehyde dehydrogenase (NAD+)	Escherichia coli	?	-	?	89	
1.2.1.99	propionaldehyde + NAD+ + H2O	-	Klebsiella pneumoniae	propionate + NADH + 2 H+	-	?	415157	
1.2.1.99	propionaldehyde + NAD+ + H2O	-	Escherichia coli	propionate + NADH + 2 H+	-	?	415157	
1.2.1.99	valeraldehyde + NAD+ + H2O	most preferred substrate	Klebsiella pneumoniae	pentanoate + NADH + H+	-	?	415430	
1.2.1.99	valeraldehyde + NAD+ + H2O	most preferred substrate	Escherichia coli	pentanoate + NADH + H+	-	?	415430