1.8.98.1 coenzyme B + 6-mercaptohexanoyl-L-threonine phosphate + methylene blue - Methanothermobacter thermautotrophicus ? + methylene blue - ir 372745 1.8.98.1 coenzyme B + 6-mercaptohexanoyl-L-threonine phosphate + methylene blue - Methanothermobacter thermautotrophicus Marburg / DSM 2133 ? + methylene blue - ir 372745 1.8.98.1 coenzyme B + coenzyme M + methanophenazine - Methanosarcina barkeri N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + dihydromethanophenazine - ? 356009 1.8.98.1 coenzyme B + coenzyme M + methanophenazine - Methanothermobacter thermautotrophicus N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + dihydromethanophenazine - ? 356009 1.8.98.1 coenzyme B + coenzyme M + methanophenazine - Methanosarcina thermophila N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + dihydromethanophenazine - ? 356009 1.8.98.1 coenzyme B + coenzyme M + methanophenazine - Methanosarcina mazei N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + dihydromethanophenazine - ? 356009 1.8.98.1 coenzyme B + coenzyme M + methanophenazine - Methanothermobacter marburgensis N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + dihydromethanophenazine - ? 356009 1.8.98.1 coenzyme B + coenzyme M + methanophenazine - Methanothermobacter thermautotrophicus Marburg / DSM 2133 N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + dihydromethanophenazine - ? 356009 1.8.98.1 coenzyme B + coenzyme M + methanophenazine - Methanococcus maripaludis N-(7-[(2-sulfoethyl)dithio]heptanoyl)-O3-phospho-L-threonine + dihydromethanophenazine - ? 413331 1.8.98.1 coenzyme B + coenzyme M + methanophenazine - Methanosarcina acetivorans N-(7-[(2-sulfoethyl)dithio]heptanoyl)-O3-phospho-L-threonine + dihydromethanophenazine - ? 413331 1.8.98.1 coenzyme B + coenzyme M + methanophenazine - Methanococcus maripaludis MM1265 N-(7-[(2-sulfoethyl)dithio]heptanoyl)-O3-phospho-L-threonine + dihydromethanophenazine - ? 413331 1.8.98.1 coenzyme B + coenzyme M + methanophenazine - Acidithiobacillus ferrooxidans N-[7-[(2-sulfoethyl)dithio]heptanoyl]-O3-phospho-L-threonine + dihydromethanophenazine - ? 440194 1.8.98.1 coenzyme B + coenzyme M + methanophenazine - Acidithiobacillus thiooxidans N-[7-[(2-sulfoethyl)dithio]heptanoyl]-O3-phospho-L-threonine + dihydromethanophenazine - ? 440194 1.8.98.1 coenzyme B + coenzyme M + methanophenazine - Acidithiobacillus thiooxidans DSM 17318 N-[7-[(2-sulfoethyl)dithio]heptanoyl]-O3-phospho-L-threonine + dihydromethanophenazine - ? 440194 1.8.98.1 coenzyme B + coenzyme M + methanophenazine - Acidithiobacillus ferrooxidans DSM 16786 N-[7-[(2-sulfoethyl)dithio]heptanoyl]-O3-phospho-L-threonine + dihydromethanophenazine - ? 440194 1.8.98.1 coenzyme B + coenzyme M + methylene blue - Methanosarcina barkeri N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + reduced methylene blue - ? 372746 1.8.98.1 coenzyme B + coenzyme M + reduced ferredoxin + H+ + coenzyme F420 - Methanosarcina acetivorans coenzyme B-coenzyme M disulfide + ferredoxin + H2 + reduced coenzyme F420 - ? 441202 1.8.98.1 coenzyme B-coenzyme M disulfide + dihydromethanophenazine - Desulfovibrio vulgaris coenzyme B + coenzyme M + methanophenazine - ? 440195 1.8.98.1 coenzyme B-coenzyme M disulfide + dihydromethanophenazine - Desulfovibrio vulgaris ATCC 29579 coenzyme B + coenzyme M + methanophenazine - ? 440195 1.8.98.1 coenzyme B-coenzyme M disulfide + reduced benzyl viologen - Methanomassiliicoccus luminyensis coenzyme B + coenzyme M + benzyl viologen - ? 441203 1.8.98.1 coenzyme B-coenzyme M disulfide + reduced benzyl viologen - Methanomassiliicoccus luminyensis DSM 25720 coenzyme B + coenzyme M + benzyl viologen - ? 441203 1.8.98.1 coenzyme B-coenzyme M disulfide + reduced ferredoxin - Methanomassiliicoccus luminyensis coenzyme B + coenzyme M + ferredoxin - ? 440196 1.8.98.1 coenzyme B-coenzyme M disulfide + reduced ferredoxin - Methanomassiliicoccus luminyensis DSM 25720 coenzyme B + coenzyme M + ferredoxin - ? 440196 1.8.98.1 coenzyme B-coenzyme M disulfide + reduced methyl viologen - Methanomassiliicoccus luminyensis coenzyme B + coenzyme M + methyl viologen - ? 441204 1.8.98.1 coenzyme B-coenzyme M disulfide + reduced methyl viologen - Methanomassiliicoccus luminyensis DSM 25720 coenzyme B + coenzyme M + methyl viologen - ? 441204 1.8.98.1 CoM-S-S-CoB + ? - Methanosarcina barkeri CoM-SH + CoB-SH + ? - ? 384461 1.8.98.1 CoM-S-S-CoB + ? - Methanosarcina thermophila CoM-SH + CoB-SH + ? - ? 384461 1.8.98.1 CoM-S-S-CoB + ? - Methanothermobacter marburgensis CoM-SH + CoB-SH + ? - ? 384461 1.8.98.1 DsrC protein disulfide + dihydromethanophenazine - Desulfovibrio vulgaris DsrC protein + methanophenazine - r 440230 1.8.98.1 DsrC protein disulfide + dihydromethanophenazine - Desulfovibrio vulgaris ATCC 29579 DsrC protein + methanophenazine - r 440230 1.8.98.1 additional information homodisulfides of coenzyme M and coenzyme B are not reduced Methanothermobacter thermautotrophicus ? - ? 89 1.8.98.1 additional information no activity with the homodisulfides of coenzyme M and coenzyme B Methanosarcina mazei ? - ? 89 1.8.98.1 additional information no activity with the homodisulfides of coenzyme M and coenzyme B Methanolobus tindarius ? - ? 89 1.8.98.1 additional information the enzyme does not catalyze the reduction of N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine with NADH, NADPH or reduced coenzyme F420 Methanothermobacter thermautotrophicus ? - ? 89 1.8.98.1 additional information Hdr complexes with F420-nonreducing hydrogenase, formate dehydrogenase, and formyl-MFR dehydrogenase, model of complex of proteins that interact with Hdr, overview Methanococcus maripaludis ? - ? 89 1.8.98.1 additional information Hdr complexes with F420-nonreducing hydrogenase, formate dehydrogenase, and formyl-MFR dehydrogenase, model of complex of proteins that interact with Hdr, overview Methanococcus maripaludis MM1265 ? - ? 89 1.8.98.1 additional information homodisulfides of coenzyme M and coenzyme B are not reduced Methanothermobacter thermautotrophicus Marburg / DSM 2133 ? - ? 89 1.8.98.1 additional information the enzyme does not catalyze the reduction of N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine with NADH, NADPH or reduced coenzyme F420 Methanothermobacter thermautotrophicus Marburg / DSM 2133 ? - ? 89 1.8.98.1 N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + coenzyme F420H2 - Methanococcus voltae coenzyme B + coenzyme M + coenzyme F420 - ? 371047 1.8.98.1 N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + coenzyme F420H2 - Methanosarcina mazei coenzyme B + coenzyme M + coenzyme F420 - ? 371047 1.8.98.1 N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + coenzyme F420H2 - Methanolobus tindarius coenzyme B + coenzyme M + coenzyme F420 - ? 371047 1.8.98.1 N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + coenzyme F420H2 reaction is coupled with the transfer of protons across the membrane of washed vesicles, which drives ATP formation from ADP Methanosarcina mazei coenzyme B + coenzyme M + coenzyme F420 - ? 371047 1.8.98.1 N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + dihydro-2-hydroxyphenazine - Methanosarcina mazei coenzyme B + coenzyme M - ? 373891 1.8.98.1 N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + dihydromethanophenazine - Methanosarcina thermophila coenzyme B + coenzyme M + methanophenazine - ? 371048 1.8.98.1 N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + dihydromethanophenazine ping-pong mechanism Methanosarcina thermophila coenzyme B + coenzyme M + methanophenazine - ? 371048 1.8.98.1 N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + H2 - Methanosarcina barkeri coenzyme B + coenzyme M - ? 371049 1.8.98.1 N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + H2 - Methanothermobacter thermautotrophicus coenzyme B + coenzyme M - ? 371049 1.8.98.1 N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + H2 - Methanosarcina mazei coenzyme B + coenzyme M - ? 371049 1.8.98.1 N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + H2 energy-conserving step Methanothermobacter thermautotrophicus coenzyme B + coenzyme M - ? 371049 1.8.98.1 N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + H2 energy-conserving step in methanohenic archaea Methanosarcina barkeri coenzyme B + coenzyme M - ? 371049 1.8.98.1 N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + H2 reaction is coupled with transfer of protons across the cytoplasmic membrane. The electrochemical proton gradient thereby generated drives ATP synthesis from ADP plus phosphate. Two different proton-translocating segments are present in the H2:heterodisulfide oxidoreductase system. The first involves the 2-hydroxyphenazine-dependent hydrogenase, and the second involves the heterodisulfide reductase Methanosarcina mazei coenzyme B + coenzyme M - ? 371049 1.8.98.1 N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + H2 reaction is independent of coenzyme F420. Reaction is coupled to proton translocation across the cytoplasmic membrane into the lumen of the everted vesicle. The transmembrane electrochemical gradient thereby generated unduces ATP synthesis from ADP + phosphate Methanosarcina mazei coenzyme B + coenzyme M - ? 371049 1.8.98.1 N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + H2 the D-enantiomer of the heterodisulfide is reduced at approximately 35% of the rate observed for the L-form Methanothermobacter thermautotrophicus coenzyme B + coenzyme M - ? 371049 1.8.98.1 N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + H2 the D-enantiomer of the heterodisulfide is reduced at approximately 35% of the rate observed for the L-form Methanothermobacter thermautotrophicus Marburg / DSM 2133 coenzyme B + coenzyme M - ? 371049 1.8.98.1 N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + H2 - Methanothermobacter thermautotrophicus Marburg / DSM 2133 coenzyme B + coenzyme M - ? 371049 1.8.98.1 N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + H2 energy-conserving step Methanothermobacter thermautotrophicus Marburg / DSM 2133 coenzyme B + coenzyme M - ? 371049 1.8.98.1 N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + reduced 2-bromophenazine - Methanosarcina mazei coenzyme B + coenzyme M + 2-bromophenazine - ? 373893 1.8.98.1 N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + reduced 2-hydroxyphenazine - Methanosarcina mazei coenzyme B + coenzyme M + 2-hydroxyphenazine - ? 373894 1.8.98.1 N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + reduced benzyl viologen - Methanosarcina barkeri coenzyme B + coenzyme M + benzyl viologen - ? 373895 1.8.98.1 N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + reduced benzyl viologen reaction intermediate is a [4Fe-4S]3+ cluster that is coordinated by one of the cysteines of nearby active-site disulfide or by the sulfur of coenzyme M Methanothermobacter marburgensis coenzyme B + coenzyme M + benzyl viologen - ? 373895 1.8.98.1 N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + reduced benzylviologen reaction intermediate is a [4Fe-4S]3+ cluster that is coordinated by one of the cysteines of nearby active-site disulfide or by the sulfur of coenzyme M Methanosarcina barkeri coenzyme B + coenzyme M + oxidized benzyl viologen - ? 373896 1.8.98.1 N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + reduced benzylviologen - Methanothermobacter thermautotrophicus coenzyme B + coenzyme M + benzylviologen - ? 373897 1.8.98.1 N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + reduced benzylviologen - Methanothermobacter thermautotrophicus coenzyme B + coenzyme M + benzylviologen - r 373897 1.8.98.1 N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + reduced cytochrome b - Methanosarcina thermophila coenzyme B + coenzyme M + oxidized cytochrome b - ? 371050 1.8.98.1 N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + reduced cytochrome b electron transfer by a CO dehydrogenase in complex with the heterodisulfide oxidoreductase, no H2 uptake in vivo, reaction is part of the overall reduction of acetate to methane and H2O, also providing a proton-translocation redox system to drive ATP formation Methanosarcina thermophila coenzyme B + coenzyme M + oxidized cytochrome b - ? 371050 1.8.98.1 N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + reduced methyl viologen ping-pong mechanism Methanosarcina thermophila coenzyme B + coenzyme M + methyl viologen - ? 373898 1.8.98.1 N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + reduced methylene blue - Methanosarcina barkeri coenzyme B + coenzyme M + methylene blue - r 373899 1.8.98.1 N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + reduced methylene blue - Methanothermobacter thermautotrophicus coenzyme B + coenzyme M + methylene blue - r 373899 1.8.98.1 N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + reduced methylviologen - Methanosarcina barkeri coenzyme B + coenzyme M + methyl viologen - ? 373900 1.8.98.1 N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + reduced methylviologen - Methanosarcina mazei coenzyme B + coenzyme M + methyl viologen - ? 373900 1.8.98.1 N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + reduced methylviologen - Methanosarcina thermophila coenzyme B + coenzyme M + methylviologen - ? 373901 1.8.98.1 N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + reduced methylviologen - Methanococcus voltae coenzyme B + coenzyme M + methylviologen - ? 373901 1.8.98.1 N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + reduced methylviologen - Methanosarcina thermophila coenzyme B + coenzyme M + oxidized methylviologen - ? 373902 1.8.98.1 N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + reduced phenazine-1-carboxylic acid - Methanosarcina mazei coenzyme B + coenzyme M + phenazine-1-carboxylic acid - ? 373903