2.4.2.48	guanine13 in tRNA(Leu) + 7-cyano-7-carbaguanine	-	Thermoplasma acidophilum	7-cyano-7-carbaguanine13 in tRNA(Leu) + guanine	-	?	443616	
2.4.2.48	guanine15 in tRNA + 7-cyano-7-carbaguanine	-	Methanosarcina acetivorans	7-cyano-7-carbaguanine15 in tRNA + guanine	-	?	419429	
2.4.2.48	guanine15 in tRNA + 7-cyano-7-carbaguanine	i.e. 7-(cyano)-7-deazaguanine = preQ0	Methanocaldococcus jannaschii	7-cyano-7-carbaguanine15 in tRNA + guanine	-	?	419429	
2.4.2.48	guanine15 in tRNA + 7-cyano-7-carbaguanine	i.e. 7-(cyano)-7-deazaguanine = preQ0. Haloferax volcanii tRNASer is used as substrate	Methanocaldococcus jannaschii	7-cyano-7-carbaguanine15 in tRNA + guanine	-	?	419429	
2.4.2.48	guanine15 in tRNA + 7-cyano-7-carbaguanine	key enzyme for the biosynthetic pathway leading to archaeosine in archaeal tRNAs	Haloferax volcanii	7-cyano-7-carbaguanine15 in tRNA + guanine	-	?	419429	
2.4.2.48	guanine15 in tRNA + 7-cyano-7-carbaguanine	archaeosine base and 7-aminomethyl-7-deazaguanine are not incorporated into tRNA by this enzyme. Does not require ATP for the base replacement reaction	Haloferax volcanii	7-cyano-7-carbaguanine15 in tRNA + guanine	-	?	419429	
2.4.2.48	guanine15 in tRNA + 7-cyano-7-carbaguanine	-	Methanosarcina acetivorans DSM 2834	7-cyano-7-carbaguanine15 in tRNA + guanine	-	?	419429	
2.4.2.48	guanine15 in tRNA + 7-cyano-7-carbaguanine	key enzyme for the biosynthetic pathway leading to archaeosine in archaeal tRNAs	Haloferax volcanii ATCC 29605	7-cyano-7-carbaguanine15 in tRNA + guanine	-	?	419429	
2.4.2.48	guanine15 in tRNA + 7-cyano-7-carbaguanine	archaeosine base and 7-aminomethyl-7-deazaguanine are not incorporated into tRNA by this enzyme. Does not require ATP for the base replacement reaction	Haloferax volcanii ATCC 29605	7-cyano-7-carbaguanine15 in tRNA + guanine	-	?	419429	
2.4.2.48	guanine15 in tRNA + 7-cyano-7-deazaguanine	-	Thermoplasma acidophilum	7-cyano-7-deazaguanine15 in tRNA + guanine	-	?	436489	
2.4.2.48	guanine15 in tRNA + 7-cyano-7-deazaguanine	-	Methanosarcina acetivorans	7-cyano-7-deazaguanine15 in tRNA + guanine	-	?	436489	
2.4.2.48	guanine15 in tRNA + 7-cyano-7-deazaguanine	-	Thermoplasma acidophilum HO-62	7-cyano-7-deazaguanine15 in tRNA + guanine	-	?	436489	
2.4.2.48	guanine15 in tRNA(Leu) + 7-cyano-7-carbaguanine	-	Pyrococcus horikoshii	7-cyano-7-carbaguanine15 in tRNA(Leu) + guanine	-	?	443617	
2.4.2.48	guanine15 in tRNA(Leu) + 7-cyano-7-carbaguanine	-	Thermoplasma acidophilum	7-cyano-7-carbaguanine15 in tRNA(Leu) + guanine	-	?	443617	
2.4.2.48	guanine15 in tRNA(Leu) + 7-cyano-7-carbaguanine	-	Pyrococcus horikoshii OT-3	7-cyano-7-carbaguanine15 in tRNA(Leu) + guanine	-	?	443617	
2.4.2.48	additional information	the enzyme catalyzes a transglycosylation reaction in which guanine is eliminated from position 15 of the tRNA and an archaeosine precursor (preQ0) is inserted. The enzyme is able to utilize both guanine and the 7-deazaguanine base preQ0 as substrates, but not other 7-deazaguanine bases, and is able to modify tRNA from all three phylogenetic domains. The enzyme shows good activity with the tRNASer transcript as well as tRNA from Escherichia coli and yeast	Methanocaldococcus jannaschii	?	-	?	89	
2.4.2.48	tRNAguanine + 7-cyano-7-deazaguanine	archaeal TGT substitutes 7-cyano-7-deazaguanine for the G in position 15 of tRNA as the first step in archaeosine formation	Pyrococcus furiosus	?	-	?	393727