2.3.1.84 - 487564, 736589 2.3.1.84 a pPTB construct is used, expression experiments are performed in Escherichia coli cells 702712 2.3.1.84 cloned from the VIN13 commercial wine yeast strain, and placed under the constitutive control of the PGK1 regulatory sequences to generate plasmids pATF2-s. Overexpression of ATF2 in vine yeast increased the concentrations of ethyl acetate and isoamyl acetate to a lesser degree than overexpression of ATF1 677273 2.3.1.84 cloned from the VIN13 commercial wine yeast strain, and placed under the constitutive control of the PGK1 regulatory sequences. Overexpression of ATF1 in vine yeast significantly increased the concentrations of ethyl acetate, isoamyl acetate, 2-phenylethyl acetate and ethyl caproate 677273 2.3.1.84 cloning of ATF1 and ATF2 genes encoding alcohol acetyltransferases 487565 2.3.1.84 cloning of ATF1 gene encoding alcohol acetyltransferase, expression in Saccharomyces cerevisiae 487560 2.3.1.84 cloning of Lg-ATF1 gene encoding alcohol acetyltransferase, expression in Saccharomyces cerevisiae 487560 2.3.1.84 cloning of the ATF1 gene encoding alcohol acetyltransferase 1 from wine yeast 487566 2.3.1.84 complete coding region of PhCFAT cDNA subcloned into expression vector pET-28a and expressed in Escherichia coli 723419 2.3.1.84 expression in Escherichia coli 720138, 735555