1.14.13.16 expressed in Escherichia coli 1.14.13.16 expression in Escherichia coli 1.14.13.16 overexpression in Escherichia coli 1.14.13.16 overexpression of wild-type and mutant enzymes in Escherichia coli 1.14.13.16 overexpression of wild-type and mutants in strains BL121(DE3) and JM109